Kovner Anna V, Kapushchak Yaroslav K, Zaparina Oxana, Mordvinov Viatcheslav A, Pakharukova Maria Y
Institute of Cytology and Genetics (ICG), Siberian Branch of Russian Academy of Sciences (SB RAS), 10 Ak. Lavrentiev Ave., Novosibirsk 630090, Russia.
Institute of Cytology and Genetics (ICG), Siberian Branch of Russian Academy of Sciences (SB RAS), 10 Ak. Lavrentiev Ave., Novosibirsk 630090, Russia; Department of Natural Sciences, Novosibirsk State University, 2 Pirogova Str., Novosibirsk 630090, Russia; Institute of Molecular Biology and Biophysics, Siberian Branch of Russian Academy of Medical Sciences, 2 Ak. Timakova Str., Novosibirsk, 630117, Russia.
Acta Trop. 2024 Feb;250:107100. doi: 10.1016/j.actatropica.2023.107100. Epub 2023 Dec 14.
The liver fluke Opisthorchis felineus is a foodborne zoonotic pathogen endemic to Russia, Kazakhstan, and several European countries. The adult flukes affect the hepatobiliary system of piscivorous mammals and humans, thereby causing numerous complications, including liver fibrosis. Detailing the mechanisms of progression of the fibrotic complications is a hot topic in the field of research on opisthorchiasis pathogenesis. Pathologic angiogenesis appears to be associated with the fibrogenic progression due to active participation in the recruitment of inflammatory cells and many factors involved in the modulation of the extracellular matrix. The aim of the study was to evaluate neoangiogenesis and amyloid deposits in liver tissues of model animals and patients with confirmed chronic opisthorchiasis. In addition, we assessed a possible correlation of neoangiogenesis with liver fibrosis. We found a significant increase in the number of newly formed vessels and amyloid deposits in the liver of people with chronic opisthorchiasis compared to that of uninfected ones. Thus, for the first time we have demonstrated neoangiogenesis and amyloid deposits during O. felineus infection in a Mesocricetus auratus model. Regression analysis showed that CD34 newly formed vessels correlate with fibrosis severity in the course of the infection. Our results indicate the potential contribution of angiogenesis to the progression of liver fibrosis, associated with O. felineus infection.
肝吸虫猫后睾吸虫是一种食源性人畜共患病原体，在俄罗斯、哈萨克斯坦和几个欧洲国家流行。成虫吸虫会影响食鱼哺乳动物和人类的肝胆系统，从而引发多种并发症，包括肝纤维化。详细阐述纤维化并发症的进展机制是后睾吸虫病发病机制研究领域的一个热门话题。病理性血管生成似乎与纤维化进展有关，因为它积极参与炎症细胞的募集以及许多参与细胞外基质调节的因子。本研究的目的是评估模型动物和确诊为慢性后睾吸虫病患者肝脏组织中的新生血管生成和淀粉样沉积。此外，我们评估了新生血管生成与肝纤维化之间可能存在的相关性。我们发现，与未感染的人相比，慢性后睾吸虫病患者肝脏中新生血管和淀粉样沉积的数量显著增加。因此，我们首次在金黄仓鼠模型中证明了猫后睾吸虫感染期间的新生血管生成和淀粉样沉积。回归分析表明，在感染过程中，CD34新生血管与纤维化严重程度相关。我们的结果表明血管生成对与猫后睾吸虫感染相关的肝纤维化进展具有潜在作用。
