Hashimoto Y, Kitagawa H S
Gan. 1979 Jun;70(3):305-14.
Antigens expressed on urinary bladder cancer cells of transplantation and tissue culture lines, which originated in tumors induced by N-butyl-N-(4-hydroxybutyl)nitrosamine in individual ACI/N rats, were studied by several immunological methods. Tumor-specific transplantation antigen was determined by transplantability of cancer cells into syngeneic rats which had been immunized with the respective cancer cells by the ligation-and-release method. Two out of 6 bladder cancer lines showed high antigenicity but antigenicities of the other 4 lines were of low or undetectable level. Cross resistance was observed in the transplantation immunity among the 2 high antigenic lines but not in the other lines. Cell-mediated cytotoxicity was assayed by the microtestplate method. The lymphoid cells from ACI rats hyper-immunized with cancer cells of a high antigenic line showed a marked cytotoxicity against cancer cells of the immunizing line but not to cells of the other bladder cancer lines including another high antigenic line that induced a cross resistance in transplantation immunity. Tumor-associated cell-surface antigen was detected by membrane immunofluorescence test with serum which was raised in allogeneic Donryu rat by the high antigenic bladder cancer and absorbed with normal ACI rat tissues. The absorbed serum gave positive membrane fluorescence to cancer cells of the immunizing line and 2 other bladder cancer lines but not to cells of other 4 bladder lines and ACI tumors other than bladder cancer. The common antigen detected by the serological method was not reflected either in transplantation immunity or cell-mediated cytotoxicity of the immune lymphoid cells.
采用多种免疫学方法，对移植瘤及组织培养细胞系的膀胱癌细胞上表达的抗原进行了研究。这些移植瘤及组织培养细胞系源自于用N-丁基-N-(4-羟丁基)亚硝胺诱发的单个ACI/N大鼠的肿瘤。通过将癌细胞移植到经结扎-释放法用相应癌细胞免疫的同基因大鼠体内，来测定肿瘤特异性移植抗原。6个膀胱癌细胞系中有2个显示出高抗原性，而其他4个细胞系的抗原性低或检测不到。在2个高抗原性细胞系之间的移植免疫中观察到交叉抗性，但在其他细胞系中未观察到。采用微量滴定板法检测细胞介导的细胞毒性。用高抗原性细胞系的癌细胞超免疫的ACI大鼠的淋巴细胞，对免疫细胞系的癌细胞显示出明显的细胞毒性，但对其他膀胱癌细胞系的细胞无细胞毒性，包括另一个在移植免疫中诱导交叉抗性的高抗原性细胞系。用高抗原性膀胱癌在同种异体唐瑞大鼠中制备并经正常ACI大鼠组织吸收的血清，通过膜免疫荧光试验检测肿瘤相关细胞表面抗原。吸收后的血清对免疫细胞系及其他2个膀胱癌细胞系的癌细胞呈阳性膜荧光反应，但对其他4个膀胱癌细胞系及除膀胱癌外的ACI肿瘤细胞无阳性反应。血清学方法检测到的共同抗原在免疫淋巴细胞的移植免疫或细胞介导的细胞毒性中均未体现。
