Masuko T, Hashimoto Y
J Natl Cancer Inst. 1981 Aug;67(2):431-6.
Inbred ACI/rats were treated with a selective urinary bladder carcinogen N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN). Two cell surface antigens on the epithelial cells of the bladder, normal bladder epithelium (NBE) antigen unique to normal epithelial cells of the bladder and BC-SK antigen specific for bladder cancer and epidermis, were periodically assayed by mixed hemadsorption test with the typing sera against each antigen. Treatment with BBN for 8 or more weeks induced papillomas or cancers that expressed a definite level of BC-SK antigen, whereas a 4-week treatment did not render the bladder cells with a significant level of this antigen. In general, the level of NBE antigen in bladder cells was not altered by the BBN treatment. In vitro culture of bladder cells of BBN-treated rats resulted in an increase in the level of BC-SK antigen as opposed to a decrease in the NBE antigen level. BC-SK antigen was not detected in epidermal cells of rats younger than 3 days, but it emerged in these cells by in vitro culture for longer than 4 days.
将近交系ACI大鼠用选择性膀胱致癌物N-丁基-N-(4-羟基丁基)亚硝胺（BBN）进行处理。通过针对每种抗原的分型血清进行混合血细胞吸附试验，定期检测膀胱上皮细胞上的两种细胞表面抗原，即膀胱正常上皮细胞（NBE）特有的正常膀胱上皮抗原和对膀胱癌及表皮具有特异性的BC-SK抗原。用BBN处理8周或更长时间可诱导出表达一定水平BC-SK抗原的乳头状瘤或癌，而处理4周则不会使膀胱细胞产生显著水平的这种抗原。一般来说，BBN处理不会改变膀胱细胞中NBE抗原的水平。BBN处理大鼠的膀胱细胞进行体外培养后，BC-SK抗原水平升高，而NBE抗原水平降低。在3日龄以内的大鼠表皮细胞中未检测到BC-SK抗原，但通过体外培养4天以上，该抗原出现在这些细胞中。
