Department of Molecular and Cellular Botany, Ruhr University Bochum, Bochum, Germany.
Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
mBio. 2024 Jan 16;15(1):e0289623. doi: 10.1128/mbio.02896-23. Epub 2023 Dec 19.
Histone chaperones are proteins that are involved in nucleosome assembly and disassembly and can therefore influence all DNA-dependent processes including transcription, DNA replication, and repair. ASF1 is a histone chaperone that is conserved throughout eukaryotes. In contrast to most other multicellular organisms, a deletion mutant of in the fungus is viable; however, the mutant is sterile. In this study, we could show that the histone-binding ability of ASF1 is required for fertility in , whereas the function of ASF1 in maintenance of genome stability does not require histone binding. We also showed that the histone modifications H3K27me3 and H3K56ac are misregulated in the Δasf1 mutant. Furthermore, we identified a large duplication on chromosome 2 of the mutant strain that is genetically linked to the Δasf1 allele present on chromosome 6, suggesting that viability of the mutant might depend on the presence of the duplicated region.
组蛋白伴侣是参与核小体组装和拆卸的蛋白质，因此可以影响所有依赖于 DNA 的过程，包括转录、DNA 复制和修复。ASF1 是一种组蛋白伴侣，在真核生物中保守存在。与大多数其他多细胞生物不同，真菌中的 缺失突变体是有活力的；然而，该突变体是不育的。在这项研究中，我们能够表明，ASF1 的组蛋白结合能力是 在 中生育所必需的，而 ASF1 在维持基因组稳定性方面的功能不需要组蛋白结合。我们还表明，在 Δasf1 突变体中，H3K27me3 和 H3K56ac 的组蛋白修饰被错误调节。此外，我们在突变株的染色体 2 上鉴定到一个大的重复序列，该重复序列与染色体 6 上存在的 Δasf1 等位基因在遗传上连锁，表明突变体的活力可能依赖于重复区域的存在。
