Trivellini Alice, Carmassi Giulia, Scatena Guido, Vernieri Paolo, Ferrante Antonio
Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy.
Italian Institute for Environmental Protection and Research - ISPRA, Via del Cedro 38, 57122, Leghorn, Italy.
Mol Hortic. 2023 Dec 19;3(1):28. doi: 10.1186/s43897-023-00075-y.
Ornamental plants are used to decorate urban and peri-urban areas, and during their cultivation or utilisation, they can be exposed to abiotic stress. Salinity is an abiotic stress factor that limits plant growth and reduces the ornamental value of sensitive species. In this study, transcriptomic analysis was conducted to identify genes associated with tolerance or sensitivity to salinity in two hibiscus (Hibiscus rosa-sinensis L.) cultivars, 'Porto' and 'Sunny wind'. The physiological and biochemical parameters of plants exposed to 50, 100, or 200 mM NaCl and water (control) were monitored. Salinity treatments were applied for six weeks. After four weeks, differences between cultivars were clearly evident and 'Porto' was more tolerant than 'Sunny wind'. The tolerant cultivar showed lower electrolyte leakage and ABA concentrations, and higher proline content in the leaves. Accumulation of Na in different organs was lower in the flower organs of 'Porto'. At the molecular level, several differential expressed genes were observed between the cultivars and flower organs. Among the highly expressed DEGs, coat protein, alcohol dehydrogenase, and AP2/EREBP transcription factor ERF-1. Among the downregulated genes, GH3 and NCED were the most interesting. The differential expression of these genes may explain the salt stress tolerance of 'Porto'.
观赏植物用于装饰城市和城郊地区，在其栽培或利用过程中，可能会受到非生物胁迫。盐度是一种限制植物生长并降低敏感物种观赏价值的非生物胁迫因素。在本研究中，进行了转录组分析，以鉴定两个芙蓉（Hibiscus rosa-sinensis L.）品种‘波尔图’和‘阳光之风’中与耐盐性或盐敏感性相关的基因。监测了暴露于50、100或200 mM NaCl和水（对照）的植物的生理生化参数。盐处理持续六周。四周后，品种间差异明显，‘波尔图’比‘阳光之风’更耐盐。耐盐品种的叶片电解质渗漏和脱落酸浓度较低，但脯氨酸含量较高‘波尔图’花器官中不同器官的Na积累较低。在分子水平上，在品种和花器官之间观察到了几个差异表达基因。在高表达的差异表达基因中，有外壳蛋白、乙醇脱氢酶和AP2/EREBP转录因子ERF-1。在下调基因中‘GH3’和‘NCED’最受关注。这些基因的差异表达可能解释了‘波尔图’的耐盐胁迫性。
