Scherer W F, Weaver S C, Taylor C A, Cupp E W, Dickerman R W, Rubino H H
Am J Trop Med Hyg. 1987 Jan;36(1):194-7. doi: 10.4269/ajtmh.1987.36.194.
The vector competence of Culex (Melanoconion) taeniopus was examined in the laboratory for "enzootic" allopatric and "epizootic" strains of Venezuelan equine encephalomyelitis viruses of hemagglutination inhibition subtypes I, II, III, and IV. Following bloodmeals from viremic hamsters, and extrinsic incubation of 20-22 days, mosquitoes were allowed to refeed for transmission attempts. Infection rates never exceeded 50% with oral doses of less than 10(4) chick embryo cell culture plaque forming units (CECPFU), and approached 100% only after ingestion of greater than or equal to 10(5.5) PFU. Transmission was achieved for some "epizootic" subtype IABC and "enzootic" subtype ID strains after bloodmeals containing greater than or equal to 10(3.4) CECPFU; subtypes II, III, and IV were never transmitted despite oral doses up to 10(5.0) CECPFU. These data contrast sharply with those reported previously for sympatric "enzootic" subtype IE Middle American Venezuelan encephalitis viruses.
在实验室中，对致倦库蚊（黑须库蚊亚属）针对血凝抑制亚型I、II、III和IV的委内瑞拉马脑炎病毒的“动物流行病”异域株和“ epizootic”株的媒介能力进行了检测。在用病毒血症仓鼠进行血餐喂养并进行20 - 22天的外在潜伏期后，让蚊子再次进食以尝试传播。口服剂量小于10(4)鸡胚细胞培养蚀斑形成单位（CECPFU）时，感染率从未超过50%，只有在摄入大于或等于10(5.5) PFU后感染率才接近100%。对于一些含有大于或等于10(3.4) CECPFU的血餐喂养后的“ epizootic” IABC亚型和“动物流行病” ID亚型毒株，实现了传播；尽管口服剂量高达10(5.0) CECPFU，但II、III和IV亚型从未传播。这些数据与先前报道的同域“动物流行病” IE亚型中美洲委内瑞拉脑炎病毒的数据形成鲜明对比。
