Kreutzer R D, Souraty N, Semko M E
Am J Trop Med Hyg. 1987 Jan;36(1):22-32. doi: 10.4269/ajtmh.1987.36.22.
An analysis was presented for identification of 20 species and subspecies of Leishmania by cellulose acetate electrophoresis data from the enzymes glucose phosphate isomerase, mannose phosphate isomerase, and phosphogluconate dehydrogenase. Most Leishmania could be identified from data of these three enzymes. The CAE data for 20 enzymes from over 300 New and Old World isolates were combined, and an analysis of the data which included calculations of genetic identities and genetic distances was reported. High levels of genetic similarity and low levels of genetic distance were noted among comparisons of local populations of the same Leishmania, and lower levels of similarity and higher levels of distance were noted among intracomplex pairings. The biochemical data suggested that similarities and differences among Leishmania could be quantified as they have been in other organisms. For the most part the data presented were consistent with the taxonomic rankings which were based on morphology, behavior, ecology, and other biochemical data.
通过葡萄糖磷酸异构酶、甘露糖磷酸异构酶和磷酸葡萄糖酸脱氢酶的醋酸纤维素电泳数据，对20种利什曼原虫物种和亚种进行了鉴定分析。大多数利什曼原虫可通过这三种酶的数据进行鉴定。综合了来自300多个新旧世界分离株的20种酶的醋酸纤维素电泳数据，并报告了对这些数据的分析，其中包括遗传同一性和遗传距离的计算。在相同利什曼原虫的本地种群比较中，发现了高水平的遗传相似性和低水平的遗传距离，而在复合体内配对中，相似性水平较低，距离水平较高。生化数据表明，利什曼原虫之间的异同可以像在其他生物体中一样进行量化。所呈现的数据在很大程度上与基于形态学、行为学、生态学和其他生化数据的分类排名一致。
