Nordvall S L, Larsson P H, Johansson S G
Allergy. 1986 Nov;41(8):575-80. doi: 10.1111/j.1398-9995.1986.tb00349.x.
Serum samples were collected from eight grass pollen hypersensitive children during a 4-year period. The sera were assayed for contents of timothy-specific IgE antibodies by RAST. Timothy-specific IgG and IgA antibodies were quantified by a refined ELISA in which covalent binding of the antigen to the polystyrene solid phase had been performed. IgG antibodies were also assayed by a Sepharose-protein-A technique with radiolabelled timothy allergens as the antigen. It was possible to register clearcut seasonal variations with postseasonally boosted antibody levels not only of timothy-specific IgE but also of IgG antibody. Both IgG1 and IgG4 antibodies specific for timothy showed seasonal variations of a similar degree. It was not possible to register seasonal variations of the same magnitude of timothy-specific IgA antibodies.
在4年的时间里，从8名对草花粉过敏的儿童中采集了血清样本。通过放射性变应原吸附试验（RAST）检测血清中梯牧草特异性IgE抗体的含量。采用一种改进的酶联免疫吸附测定法（ELISA）对梯牧草特异性IgG和IgA抗体进行定量，该方法中抗原已与聚苯乙烯固相进行共价结合。还采用琼脂糖蛋白A技术，以放射性标记的梯牧草过敏原作为抗原检测IgG抗体。不仅梯牧草特异性IgE抗体，而且IgG抗体在季后抗体水平升高，都有可能记录到明显的季节性变化。对梯牧草特异的IgG1和IgG4抗体均显示出相似程度的季节性变化。无法记录到梯牧草特异性IgA抗体相同幅度的季节性变化。
