基于卡波姆的乳胶凝胶中（Khip）提取物的包封及其增强的抗氧化作用和体外评价

Encapsulation of (Khip) Extract in Carbomer Based Emulgel for Its Enhanced Antioxidant Effects and Its In Vitro Evaluation.

作者信息

Masood Shamaila, Arshad Muhammad Sohail, Khan Haji Muhammad Shoaib, Begum M Yasmin, Khan Kashif Ur Rehman

机构信息

Department of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakriya University, Multan 60800, Pakistan.

Department of Pharmaceutics, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.

出版信息

Gels. 2023 Dec 13;9(12):977. doi: 10.3390/gels9120977.

DOI:10.3390/gels9120977

PMID:38131963

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10743248/

Abstract

BACKGROUND

The use of natural products in skin care has been valued for their tremendous therapeutic benefits since ancient times. The current study was aimed at exploring the plant extract and development of a stable emulgel loaded with the same extract to assess its cosmeceutical potentials.

METHODOLOGY

A stable emulgel loaded with methanolic plant extract along with its control gel was prepared by homogenization. The antioxidant potential of extracts prepared in different solvents (methanol MLP, ethanol ELP, n-hexane nLP, ethyl acetate EALP, and petroleum ether PLP) was determined by DPPH scavenging activity. The presence of phytochemicals was confirmed by total phenolic and flavonoid content analysis (TPC/TFC). HPLC was used for quantification of bioactive components. FTIR analysis was performed for confirmation of functional groups. SPF was calculated via spectroscopic analysis for extract, control gel, and extract loaded emulgel. Stability studies included physical evaluation, pH, conductivity, spreadability, and rheological testing of both control and test emulgels at different temperatures, i.e., 8 °C ± 1, 25 °C ± 1, 40 °C ± 1, 40 °C ± 1 with RH of 75% for a period of 90 days.

RESULTS

DPPH radical scavenging activity showed the highest antioxidant activity of 85.5% ± 2.78 for MLP. TPC and TFC were also found to be highest for the methanolic fraction, i.e., 190.98 ± 0.40 mgGAE/g and 128.28 ± 2.64 mgQE/g, respectively. The SPF of methanolic extract, placebo gel, and LPEG was 13.43 ± 0.46, 2.37 ± 0.33, and 7.28 ± 0.56, respectively. HPLC assay confirmed the presence of catechin, vanillic acid, caffeic acid, and sinapinic acid. Rheological analysis showed that formulation has pseudo-plastic flow behavior. Other stability tests also revealed that prepared emulgel is a stable one.

CONCLUSION

A stable emulgel loaded with plant extract was successfully prepared and characterized for its cosmetic effects.

摘要

背景

自古以来，天然产物在皮肤护理中的应用因其巨大的治疗功效而受到重视。本研究旨在探索植物提取物，并开发一种负载相同提取物的稳定乳胶凝胶，以评估其药妆潜力。

方法

通过均质法制备了一种负载甲醇植物提取物的稳定乳胶凝胶及其对照凝胶。通过DPPH清除活性测定了在不同溶剂（甲醇MLP、乙醇ELP、正己烷nLP、乙酸乙酯EALP和石油醚PLP）中制备的提取物的抗氧化潜力。通过总酚和黄酮含量分析（TPC/TFC）确认植物化学物质的存在。采用高效液相色谱法对生物活性成分进行定量。通过傅里叶变换红外光谱分析确认官能团。通过光谱分析计算提取物、对照凝胶和负载提取物的乳胶凝胶的防晒系数（SPF）。稳定性研究包括在不同温度（即8℃±1、25℃±1、40℃±1、40℃±1，相对湿度75%）下对对照乳胶凝胶和测试乳胶凝胶进行90天的物理评估、pH值、电导率、铺展性和流变学测试。

结果

DPPH自由基清除活性显示，MLP的抗氧化活性最高，为85.5%±2.78。甲醇提取物的总酚和黄酮含量也最高，分别为190.98±0.40mg GAE/g和128.28±2.64mg QE/g。甲醇提取物、安慰剂凝胶和LPEG的SPF分别为13.43±0.46、2.37±0.33和7.28±0.56。高效液相色谱分析证实了儿茶素、香草酸、咖啡酸和芥子酸的存在。流变学分析表明，该制剂具有假塑性流动行为。其他稳定性测试还表明，制备的乳胶凝胶是稳定的。