Lee Sunny Chung, Ahn Jongmin, Kim Jina, Lee Joo-Yeon, Kim Juhae, Uddin Md Salah, Lee Sang Woo, Kim Choon Young
Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095-1735, USA.
Natural Product Central Bank & Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Chungbuk, Republic of Korea.
Antioxidants (Basel). 2023 Nov 23;12(12):2037. doi: 10.3390/antiox12122037.
Hallier f. (MU) has been used as an anti-inflammatory agent to treat burns and scales. However, the potential anti-inflammatory mechanisms of action of this plant have not been elucidated. This study aimed to assess the antioxidant and anti-inflammatory effects of the leaf and shoot of MU grown in Bangladesh. The MU extract exhibited antioxidant activities as demonstrated by DPPH and ABTS free-radical-scavenging activities and the total polyphenol and total flavonoid contents. MU extract significantly reduced the lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in RAW264.7 macrophage. Accordingly, the gene levels of inducible NO synthase and cyclooxygenase-2 were suppressed. The MU extract alleviated the LPS-induced expression of TLR4, NF-κB, and inflammatory cytokines (, , and ). The constituents of a MU extract were tentatively identified using UHPLC-PDA-QTOF/MS techniques. The main compounds were identified as 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, quercitrin, and 4,5-dicaffeoylquinic acid. Molecular docking analysis revealed that these compounds interact with TLR4 protein, with quercitrin showing the highest binding affinity among them. Overall, our findings demonstrate the antioxidant and in vitro anti-inflammatory activities of MU and its potential compounds to target the TLR4-NF-κB signaling pathway. These findings are potentially used to further explore promising natural food ingredients that are effective in regulating inflammation.
哈利尔氏蕨（MU）已被用作抗炎剂来治疗烧伤和鳞屑。然而，这种植物潜在的抗炎作用机制尚未阐明。本研究旨在评估在孟加拉国种植的MU的叶和茎的抗氧化和抗炎作用。MU提取物表现出抗氧化活性，如通过DPPH和ABTS自由基清除活性以及总多酚和总黄酮含量所证明的那样。MU提取物显著降低了脂多糖（LPS）刺激的RAW264.7巨噬细胞中一氧化氮（NO）的产生。相应地，诱导型NO合酶和环氧化酶-2的基因水平受到抑制。MU提取物减轻了LPS诱导的TLR4、NF-κB和炎性细胞因子（、和）的表达。使用UHPLC-PDA-QTOF/MS技术初步鉴定了MU提取物的成分。主要化合物被鉴定为3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、槲皮苷和4,5-二咖啡酰奎宁酸。分子对接分析表明，这些化合物与TLR4蛋白相互作用，其中槲皮苷表现出最高的结合亲和力。总体而言，我们的研究结果证明了MU及其潜在化合物针对TLR4-NF-κB信号通路的抗氧化和体外抗炎活性。这些发现可能用于进一步探索在调节炎症方面有效的有前景的天然食品成分。
