College of Plant Protection, Yangzhou University, Yangzhou 225009, China.
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China.
Genes (Basel). 2023 Nov 30;14(12):2165. doi: 10.3390/genes14122165.
The rice leaf folder, (Lepidoptera: Pyralidae), is a notorious pest of rice in Asia. The larvae and adults of utilize specialized chemosensory systems to adapt to different environmental odors and physiological behaviors. However, the differences in chemosensory genes between the olfactory organs of these two different developmental stages remain unclear. Here, we conducted a transcriptome analysis of larvae heads, male antennae, and female antennae in and identified 131 putative chemosensory genes, including 32 s (8 novel s), 23 s (2 novel s), 55 s (17 novel s), 19 s (5 novel s) and 2 s. Comparisons between larvae and adults of by transcriptome and RT-qPCR analysis revealed that the number and expression of chemosensory genes in larval heads were less than that of adult antennae. Only 17 chemosensory genes (7 s and 10 s) were specifically or preferentially expressed in the larval heads, while a total of 101 chemosensory genes (21 s, 9 s, 51 s, 18 s, and 2 s) were specifically or preferentially expressed in adult antennae. Our study found differences in chemosensory gene expression between larvae and adults, suggesting their specialized functions at different developmental stages of . These results provide a theoretical basis for screening chemosensory genes as potential molecular targets and developing novel management strategies to control .
稻纵卷叶螟（Lepidoptera: Pyralidae）是亚洲水稻的一种恶名昭彰的害虫。幼虫和成虫利用专门的化感感觉系统来适应不同的环境气味和生理行为。然而，这两个不同发育阶段的嗅觉器官中的化感感觉基因的差异尚不清楚。在这里，我们对稻纵卷叶螟幼虫头部、雄触角和雌触角进行了转录组分析，鉴定出了 131 个可能的化感感觉基因，包括 32 个 s（8 个新 s）、23 个 s（2 个新 s）、55 个 s（17 个新 s）、19 个 s（5 个新 s）和 2 个 s。通过转录组和 RT-qPCR 分析比较幼虫和成虫的结果表明，幼虫头部的化感感觉基因数量和表达水平均低于成虫触角。只有 17 个化感感觉基因（7 个 s 和 10 个 s）在幼虫头部特异性或优先表达，而总共 101 个化感感觉基因（21 个 s、9 个 s、51 个 s、18 个 s 和 2 个 s）在成虫触角中特异性或优先表达。我们的研究发现幼虫和成虫之间化感感觉基因表达存在差异，这表明它们在不同发育阶段具有专门的功能。这些结果为筛选化感感觉基因作为潜在的分子靶标和开发新的管理策略来控制稻纵卷叶螟提供了理论基础。
