Zhang Yadan, Yang Rong, Yuan Guangying, Li Weidong, Cui Zihao, Xiao Zhuangzhuang, Dong Xiaofei, Yang Hongqiang, Liu Xiaojuan, Zhang Le, Hou Yirong, Liu Manyu, Liu Sushi, Hao Yu, Zhang Yuntao, Zheng Xiaotong
Beijing Institute of Biological Products Company Limited, Beijing 100170, China.
Vaccines (Basel). 2023 Nov 22;11(12):1744. doi: 10.3390/vaccines11121744.
There are some concerns about the safety of live attenuated yellow fever vaccines (YF-live), particularly viscerotropic adverse events, which have a high mortality rate. The cellular production of the vaccine will not cause these adverse effects and has the potential to extend applicability to those who have allergic reactions, immunosuppression, and age. In this study, inactivated yellow fever (YF) was prepared and adsorbed with Alum/CpG. The cellular and humoral immunities were investigated in a mouse model. The results showed that Alum/CpG (20 μg/mL) could significantly increase the binding and neutralizing activities of the antibodies against YF. Moreover, the antibody level at day 28 after one dose was similar to that of the attenuated vaccine, but significantly higher after two doses. At the same time, Alum/CpG significantly increased the levels of IFN-γ and IL-4 cytokines.
对于减毒活黄热病疫苗（YF-live）的安全性存在一些担忧，尤其是嗜内脏性不良事件，其死亡率很高。疫苗的细胞生产不会引起这些不良反应，并且有可能扩大适用范围至有过敏反应、免疫抑制和特定年龄的人群。在本研究中，制备了灭活黄热病（YF）并用明矾/CpG进行吸附。在小鼠模型中研究了细胞免疫和体液免疫。结果表明，明矾/CpG（20μg/mL）可显著提高抗YF抗体的结合和中和活性。此外，一剂后第28天的抗体水平与减毒疫苗相似，但两剂后显著更高。同时，明矾/CpG显著提高了IFN-γ和IL-4细胞因子的水平。
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