评价多肽-p 在酿酒酵母中克隆、表达和分泌对糖尿病大鼠的影响。
Evaluation of the impact of polypeptide-p on diabetic rats upon its cloning, expression, and secretion in Saccharomyces boulardii.
机构信息
Department of Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Genetic Engineering and Molecular Genetics, Gorgan University of Agricultural Science and Natural Resources, P.O. Box: 4934174515, Gorgan, Iran.
出版信息
Arch Microbiol. 2023 Dec 23;206(1):37. doi: 10.1007/s00203-023-03773-9.
This study was designed to evaluate the effectiveness of recombinant polypeptide-p derived from Momordica charantia on diabetic rats. In this research, the optimized sequence of polypeptide-p gene fused to a secretion signal tag was cloned into the expression vector and transformed into probiotic Saccharomyces boulardii. The production of recombinant secretion protein was verified by western blotting, HPLC, and mass spectrometry. To assay recombinant yeast bioactivity in the gut, diabetic rats were orally fed wild-type and recombinant S. boulardii, in short SB and rSB, respectively, at two low and high doses as well as glibenclamide as a reference drug. In untreated diabetic and treated diabetic + SB rats (low and high doses), the blood glucose increased from 461, 481, and 455 (mg/dl), respectively, to higher than 600 mg/dl on the 21st day. Whereas glibenclamide and rSB treatments showed a significant reduction in the blood glucose level. The result of this study promised a safe plant-source supplement for diabetes through probiotic orchestration.
本研究旨在评估苦瓜衍生重组多肽-p 对糖尿病大鼠的有效性。在这项研究中，将融合了分泌信号标签的多肽-p 基因的优化序列克隆到表达载体中，并转化为益生菌酿酒酵母。通过 Western blot、HPLC 和质谱验证重组分泌蛋白的产生。为了检测重组酵母在肠道中的生物活性，将野生型和重组酿酒酵母（分别为 SB 和 rSB）以及格列本脲作为参考药物以低剂量和高剂量分别口服给予糖尿病大鼠。在未经治疗的糖尿病和治疗的糖尿病+SB 大鼠（低剂量和高剂量）中，血糖分别从 461、481 和 455（mg/dl）升高到 600 mg/dl 以上，第 21 天。而格列本脲和 rSB 治疗则显著降低了血糖水平。这项研究的结果为通过益生菌协调为糖尿病提供了一种安全的植物来源补充剂。
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