Resistance and inhibitor testing on (Linnaeus) (Culicidae: Diptera) populations in the Florida Keys.

is the species of greatest concern for mosquito-borne disease in the Florida Keys. Previous locally transmitted dengue outbreaks in Key West (2009-2010) and Key Largo (2020) illustrate the need for an immediate and effective response plan to maintain populations below threshold levels. An important part of the Florida Keys Mosquito Control District's vector response plan is adulticide application because it can provide an immediate reduction in adults in the community. It has become apparent that in the Florida Keys, and throughout Florida, resistance to the adulticide permethrin is prevalent. This study uses the CDC bottle bioassay method to look at resistance in collected from Key Largo, Vaca Key, and Key West, FL. Resistance was found in all three populations when exposed to permethrin and Sumithrin® but not malathion. Inhibitor testing revealed that esterase and glutathione transferase activity is involved in resistance to permethrin in Key Largo and Key West populations while oxidase activity is involved in resistance to permethrin in from Vaca Key. Lack of knockdown at the diagnostic time and previous studies detecting the presence of -associated allele mutations suggest knockdown resistance in all three populations. Results from this study show that there are multiple factors involved with resistance in the populations in the Florida Keys and that resistance mechanisms vary between islands. Continued surveillance will remain important so the most effective active ingredients can be used in response to future disease transmission.