Astuti Suryani Dyah, Febriastri Amalia Rizky, Mukti Akhmad Taufiq, Yaqubi Ahmad Khalil, Susilo Yunus, Syahrom Ardiansyah
Department of Physics, Faculty of Sciences and Technology, Airlangga University, 60115, Surabaya, Indonesia.
Faculty of Sciences and Technology, Airlangga University, 60115, Surabaya, Indonesia.
Heliyon. 2023 Dec 3;9(12):e23253. doi: 10.1016/j.heliyon.2023.e23253. eCollection 2023 Dec.
The purpose of this study is to determine how photo biomodulation therapy utilizing infrared diode laser irradiation (975.2 nm) affects the gonadal maturity level (GML) of male Siamese catfish (Pan-gasianodon hypothalamus). The interest in applying laser therapy in medicine and dentistry has remarkably increased in the last decade. Different types of lasers are available, and their usage is well-defined by different parameters, such as wavelength, energy density, power output, duration of radiation, power density and radiation mode. Infrared diode laser irradiation is used at the reproductive point (governor's vessel), situated 2/3 of the way between the anus and the pectoral fin. This study examined the metrics GML, gonads somatic index, and hepatosomatic index. The treatments were Control+ (ovaprim), Control- (without the treatment), P1 (0.2 J/cm), P2 (0.4 J/cm), P3 (0.6 J/cm), and P4 (0.8 J/cm). Therapy with infrared diode laser irradiation can modify gonad maturity (GML), gonadosomal index, and hepatosomatic index in male Siamese catfish. The photobiomodulation effect of an infrared laser stimulated the gonadal maturation of Siamese catfish. This is based on the values of wavelength (nm), power (mW), beam area (cm), time (s), radiation mode (rad) and energy dose (J/cm) in Control- (no treatment), control+ (ovaprim), P1, P2, P3, and P4. The increase in the observed parameter values is due to the vitellogenesis process. The fish gonads at the GML IV had the highest GML at P2 (dose 0.4 J/cm), with a GSI value of 1.02% and an HSI value of 1.46%. According to the study's findings, photo biomodulation therapy with infrared diode laser exposure at a dose of 0.4 J/cm is the best way to increase the gonad maturity of male Siamese catfish.
本研究的目的是确定利用红外二极管激光照射（975.2纳米）的光生物调节疗法如何影响雄性暹罗鲶鱼（苏氏圆腹鱼）的性腺成熟水平（GML）。在过去十年中，激光疗法在医学和牙科领域的应用兴趣显著增加。有不同类型的激光，其使用由不同参数明确界定，如波长、能量密度、功率输出、辐射持续时间、功率密度和辐射模式。红外二极管激光照射用于生殖点（督脉），位于肛门和胸鳍之间2/3处。本研究检测了性腺成熟水平、性腺体指数和肝体指数等指标。处理组包括Control+（使用促排卵素）、Control-（未处理）、P1（0.2焦耳/平方厘米）、P2（0.4焦耳/平方厘米）、P3（0.6焦耳/平方厘米）和P4（0.8焦耳/平方厘米）。红外二极管激光照射疗法可改变雄性暹罗鲶鱼的性腺成熟度（GML）、性腺体指数和肝体指数。红外激光的光生物调节作用刺激了暹罗鲶鱼的性腺成熟。这是基于Control-（未处理）、Control+（使用促排卵素）、P1、P2、P3和P4中的波长（纳米）、功率（毫瓦）、光束面积（平方厘米）、时间（秒）、辐射模式（拉德）和能量剂量（焦耳/平方厘米）值。观察到的参数值增加是由于卵黄发生过程。处于性腺成熟水平IV期的鱼性腺在P2（剂量0.4焦耳/平方厘米）时性腺成熟度最高，性腺体指数值为1.02%，肝体指数值为1.46%。根据该研究结果，以0.4焦耳/平方厘米的剂量进行红外二极管激光照射的光生物调节疗法是提高雄性暹罗鲶鱼性腺成熟度的最佳方法。
