Dumani Aysin, Yoldas Oguz, Yilmaz Sehnaz, Koksal Fatih, Kayar Begum, Akcimen Beril, Seydaoglu Gulsah
DDS, PhD. Assistant Professor, Cukurova University, Faculty of Dentistry, Department of Endodontics.
DDS, PhD. Professor, Cukurova University, Faculty of Dentistry, Department of Endodontics.
J Clin Exp Dent. 2012 Feb 1;4(1):e34-9. doi: 10.4317/jced.50669. eCollection 2012 Feb.
The aim of this study was to determine the frequency of two important pathogenic microorganisms associated with endodontic infections, Enterococcus faecalis and Candida albicans, in root canal samples from patients with necrotic pulps or failed canal therapy by polymerase chain reaction method.
Microbial samples were obtained from 117 teeth with necrotic pulp tissues and 114 teeth with failed endodontic treatment.
E.faecalis were identified in 16% of the necrotic and 10% of the retreated root canal infections by PCR. C.albicans genome were identified in 20% and 11% of the necrotic and retreated root canal infections, respectively, by PCR. The frequencies of microbiota were not statistically different between necrotic and retreatment groups (p > 0.05, chi squared test).
PCR analysis of teeth with periapical lesions revealed that E.faecalis was found in fewer patients than in previous studies. The C.albicans prevelance was consistent with previous reports. No statistical difference was found between primary and secondary root canal infections for C.albicans or E.faecalis. Key words:Primary root canal infection, secondary root canal infection, E.faecalis, C.albicans.
本研究旨在通过聚合酶链反应方法，确定与牙髓感染相关的两种重要致病微生物——粪肠球菌和白色念珠菌，在坏死牙髓患者或根管治疗失败患者根管样本中的出现频率。
从117颗有坏死牙髓组织的牙齿和114颗根管治疗失败的牙齿中获取微生物样本。
通过聚合酶链反应，在16%的坏死根管感染和10%的再治疗根管感染中鉴定出粪肠球菌。通过聚合酶链反应，分别在20%的坏死根管感染和11%的再治疗根管感染中鉴定出白色念珠菌基因组。坏死组和再治疗组之间微生物群的频率无统计学差异（p>0.05，卡方检验）。
对根尖周病变牙齿的聚合酶链反应分析显示，粪肠球菌在患者中的发现率低于以往研究。白色念珠菌的患病率与以往报告一致。白色念珠菌或粪肠球菌在原发性和继发性根管感染之间未发现统计学差异。关键词：原发性根管感染、继发性根管感染、粪肠球菌、白色念珠菌
