Institute for Sustainable Plant Protection, National Research Council, Strada delle Cacce 73, Turin, Italy.
Institute for Sustainable Plant Protection, National Research Council, Via Madonna del Piano 10, Sesto Fiorentino, Italy.
Planta. 2023 Dec 30;259(2):33. doi: 10.1007/s00425-023-04310-0.
Root transcriptomics and biochemical analyses in water-stressed Pisum sativum plants inoculated with Pseudomonas spp. suggested preservation of ABA-related pathway and ROS detoxification, resulting in an improved tolerance to stress. Drought already affects agriculture in large areas of the globe and, due to climate change, these areas are predicted to become increasingly unsuitable for agriculture. For several years, plant growth-promoting bacteria (PGPB) have been used to improve legume yields, but many aspects of this interaction are still unclear. To elucidate the mechanisms through which root-associated PGPB can promote plant growth in dry environments, we investigated the response of pea plants inoculated with a potentially beneficial Pseudomonas strain (PK6) and subjected to two different water regimes. Combined biometric, biochemical, and root RNA-seq analyses revealed that PK6 improved pea growth specifically under water deficit, as inoculated plants showed an increased biomass, larger leaves, and longer roots. Abscisic acid (ABA) and proline quantification, together with the transcriptome analysis, suggested that PK6-inoculated plant response to water deficit was more diversified compared to non-inoculated plants, involving alternative metabolic pathways for the detoxification of reactive oxygen species (ROS) and the preservation of the ABA stress signaling pathway. We suggest that the metabolic response of PK6-inoculated plants was more effective in their adaptation to water deprivation, leading to their improved biometric traits. Besides confirming the positive role that PGPB can have in the growth of a legume crop under adverse conditions, this study offers novel information on the mechanisms regulating plant-bacteria interaction under varying water availability. These mechanisms and the involved genes could be exploited in the future for the development of legume varieties, which can profitably grow in dry climates.
在接种了假单胞菌的豌豆植物中进行的根转录组学和生化分析表明，ABA 相关途径和 ROS 解毒得到了保护，从而提高了对胁迫的耐受性。干旱已经影响到全球大片地区的农业，而且由于气候变化，这些地区预计将变得越来越不适合农业。多年来，植物促生细菌（PGPB）一直被用于提高豆科作物的产量，但这种相互作用的许多方面仍不清楚。为了阐明与根相关的 PGPB 如何在干燥环境中促进植物生长的机制，我们研究了接种了一种潜在有益的假单胞菌（PK6）并受到两种不同水分条件的豌豆植物的反应。综合生物计量学、生物化学和根 RNA-seq 分析表明，PK6 特别在水分亏缺条件下改善了豌豆的生长，因为接种植物的生物量增加、叶片更大、根系更长。ABA 和脯氨酸的定量分析，以及转录组分析表明，与未接种植物相比，PK6 接种植物对水分亏缺的反应更加多样化，涉及到用于解毒活性氧（ROS）和保护 ABA 应激信号通路的替代代谢途径。我们认为，PK6 接种植物的代谢反应在适应水分胁迫方面更为有效，从而改善了它们的生物计量特征。除了证实 PGPB 在不利条件下对豆科作物生长的积极作用外，本研究还提供了关于在不同水分供应下调节植物-细菌相互作用的机制的新信息。这些机制和涉及的基因可以在未来用于开发能够在干旱气候中生长的豆科作物品种。
