Key Laboratory of Crops Physiology, Ecology and Genetic Breeding, Ministry of Education/College of Agronomy, Jiangxi Agricultural University, 330045, Nanchang, China.
Department of Chemistry, Al-Leith University College, Umm Al Qura University, Makkah, Saudi Arabia.
Mol Biol Rep. 2022 Jun;49(6):5595-5609. doi: 10.1007/s11033-022-07529-4. Epub 2022 May 18.
Legumes are an imperative source of food and proteins across the globe. They also improve soil fertility through symbiotic nitrogen fixation (SNF). Genome editing (GE) is now a novel way of developing desirable traits in legume crops. Genome editing tools like clustered regularly interspaced short palindromic repeats (CRISPR) system permits a defined genome alteration to improve crop performance. This genome editing tool is reliable, cost-effective, and versatile, and it has to deepen in terms of use compared to other tools. Recently, many novel variations have drawn the attention of plant geneticists, and efforts are being made to develop trans-gene-free cultivars for ensuring biosafety measures. This review critically elaborates on the recent development in genome editing of major legumes crops. We hope this updated review will provide essential informations for the researchers working on legumes genome editing. In general, the CRISPR/Cas9 novel GE technique can be integrated with other techniques like omics approaches and next-generation tools to broaden the range of gene editing and develop any desired legumes traits. Regulatory ethics of CRISPR/Cas9 are also discussed.
豆类是全球重要的食物和蛋白质来源。它们还通过共生固氮（SNF）提高土壤肥力。基因组编辑（GE）现在是在豆科作物中开发理想性状的一种新方法。基因组编辑工具，如成簇规律间隔短回文重复（CRISPR）系统，允许对基因组进行定义性的改变，以提高作物的性能。与其他工具相比，这种基因组编辑工具具有可靠性、成本效益和多功能性，需要在使用方面进一步深化。最近，许多新的变化引起了植物遗传学家的关注，正在努力开发无转基因品种，以确保生物安全措施。本综述批判性地阐述了主要豆科作物基因组编辑的最新发展。我们希望这篇最新的综述能为从事豆类基因组编辑的研究人员提供重要信息。一般来说，CRISPR/Cas9 新型 GE 技术可以与其他技术（如组学方法和下一代工具）相结合，扩大基因编辑的范围，开发任何所需的豆类特性。还讨论了 CRISPR/Cas9 的监管伦理。
