School of Life Sciences, Nantong University, 226019 Nantong, China.
ACS Appl Mater Interfaces. 2023 Sep 6;15(35):41385-41402. doi: 10.1021/acsami.3c09237. Epub 2023 Aug 22.
Effective repair and functional recovery of large peripheral nerve deficits are urgent clinical needs. A biofunctional electroactive scaffold typically acts as a "bridge" for the repair of large nerve defects. In this study, we constructed a biomimetic piezoelectric and conductive aligned polypyrrole (PPy)/polydopamine (PDA)/poly-l-lactic acid (PLLA) electrospun fibrous scaffold to improve the hydrophilicity and cellular compatibility of PLLA and restore the weakened piezoelectric effect of PDA, which is beneficial in promoting Schwann cell differentiation and dorsal root ganglion neuronal extension and alignment. The aligned PPy/PDA/PLLA fibrous scaffold bridged the sciatic nerve of Sprague-Dawley rats with a 10 mm deficit, prevented autotomy, and promoted nerve regeneration and functional recovery, thereby activating the calcium and AMP-activated protein kinase signaling pathways. Therefore, electroactive fibrous scaffolds exhibit great potential for neural tissue regeneration.
有效修复和功能恢复大的周围神经缺损是迫切的临床需求。生物功能电活性支架通常充当大神经缺损修复的“桥梁”。在这项研究中，我们构建了一种仿生压电和导电取向聚吡咯（PPy）/聚多巴胺（PDA）/聚左旋乳酸（PLLA）静电纺丝纤维支架，以提高 PLLA 的亲水性和细胞相容性，并恢复 PDA 减弱的压电效应，这有利于促进施万细胞分化和背根神经节神经元的延伸和排列。取向 PPy/PDA/PLLA 纤维支架桥接了 10mm 缺损的 Sprague-Dawley 大鼠坐骨神经，防止了自截，并促进了神经再生和功能恢复，从而激活了钙和 AMP 激活的蛋白激酶信号通路。因此，电活性纤维支架在神经组织再生方面具有巨大的潜力。
