Department of Urology, Affiliated Wuxi No.2 Hospital, Nantong University, Wuxi, China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Front Immunol. 2022 Jun 16;13:843695. doi: 10.3389/fimmu.2022.843695. eCollection 2022.
Mounting evidence suggests that bacterial dysbiosis and immunity disorder are associated with patients with chronic kidney disease (CKD), but the mycobiome is beginning to gain recognition as a fundamental part of our microbiome. We aim to characterize the profile of the mycobiome in the gut of CKD patients and its correlation to serum immunological profiles.
Ninety-two CKD patients and sex-age-body mass index (BMI)-matched healthy controls (HCs) were recruited. Fresh samples were collected using sterile containers. ITS transcribed spacer ribosomal RNA gene sequencing was performed on the samples. An immunoturbidimetric test was used to assess the serum levels of immunological features.
The CKD cohort displayed a different microbial community from that in the HC cohort according to principal coordinate analysis (PCoA). (=0.001). The comparison of the two cohorts showed that the CKD cohort had significantly higher gut microbial richness and diversity (<0.05). The CKD cohort had lower abundances of , , , and compared to the HC cohort, while it had higher (<0.05). However, the microbial community alteration was inconsistent with the severity of kidney damage in patients, as only patients in CKD stage 1~3 had differed microbial community concerning for HCs based on PCoA (<0.05). The serum concentration of the kappa light chain in CKD patients was positively associated with , whereas the it was negatively associated with (<0.05).
Not only was gut mycobiome dysbiosis observed in CKD patients, but the dysbiosis was also associated with the immunological disorder. These findings suggest that therapeutic strategies targeting gut mycobiome might be effective.
越来越多的证据表明，细菌失调和免疫紊乱与慢性肾脏病（CKD）患者有关，但真菌群开始被认为是我们微生物组的一个基本组成部分。我们旨在描述 CKD 患者肠道中真菌组的特征及其与血清免疫学特征的相关性。
招募了 92 名 CKD 患者和性别年龄-体重指数（BMI）匹配的健康对照（HCs）。使用无菌容器采集新鲜样本。对样本进行 ITS 转录间隔核糖体 RNA 基因测序。采用免疫比浊法检测血清免疫学特征水平。
根据主坐标分析（PCoA），CKD 组的微生物群落与 HC 组不同（=0.001）。两组比较显示，CKD 组肠道微生物丰富度和多样性显著高于 HC 组（<0.05）。与 HC 组相比，CKD 组的 、 、 和 丰度较低，而 丰度较高（<0.05）。然而，微生物群落的改变与患者肾脏损伤的严重程度不一致，因为只有 CKD 1~3 期的患者与 HC 相比，其微生物群落发生了改变（基于 PCoA，<0.05）。CKD 患者血清中κ轻链的浓度与 呈正相关，而与 呈负相关（<0.05）。
不仅在 CKD 患者中观察到肠道真菌失调，而且失调与免疫紊乱有关。这些发现表明，针对肠道真菌组的治疗策略可能是有效的。
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