Boushaba Nadjet, Tabet-Aoul Nacera
Laboratory Genetics Molecular and Cellular, Department of Molecular Genetics Applied, Faculty of Nature and Life Sciences, University of Science and Technology of Oran "Mohamed Boudiaf ", B.P 1505 El M'Naouar, 31000 Oran, Algeria.
Department of Biotechnology, Faculty of Nature and Life Sciences, University of Oran 1 "Ahmed Ben Bella", 31000 Oran, Algeria.
Mol Biol Res Commun. 2024;13(1):43-49. doi: 10.22099/mbrc.2023.47661.1841.
Milk protein genetic polymorphisms are associated with economically important traits in dairy cattle. The objective of this study is to genotype a single nucleotide polymorphism (SNP) responsible for the amino acid changes in the beta-lactoglobulin (β-Lg) variants A and B on 85 unrelated DNA representing Algerian cattle populations: Chelifienne (28), Cheurfa (31) and Guelmoise (26). The method used is the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). Genetic polymorphism was detected by digestion of PCR products amplified of exon II of β-Lg gene by with the endonuclease III enzyme. The results revealed that the amplified product was observed as 247 bp. Restriction digestion with III revealed three genotypes: AA, AB and BB. The genotypic frequencies of AA, AB and BB genotypes were 0.08, 0.41, 0.50; 0.08, 0.41, 0.50 and 0.01, 0.19, 0.56 in Chelifienne, Cheurfa and Guelmoise and respectively. Frequency of AA genotype was absent in Guelmoise population. Frequencies of A and B alleles were 0.29 and 0.71 in both Chelifienne and Cheurfa and 0.25 and 0.75 Guelmoise population. These results further confirm that Bos torus cattle are predominantly of β-Lactoglobulin B type. The Chi-square test at -value < 0.05 results revealed that the Chelifienne and Cheurfa populations were in Hardy-Weinberg equilibrium and the results are not significant for the Guelmoise. This genetic information could be useful to estimate the effect of polymorphism on different milk production of Algerian bovine populations.
乳蛋白基因多态性与奶牛的重要经济性状相关。本研究的目的是对85份代表阿尔及利亚牛群（谢利菲安牛28份、舍尔法牛31份和盖尔莫瓦牛26份）的无关DNA样本进行基因分型，该单核苷酸多态性（SNP）导致β-乳球蛋白（β-Lg）A和B变体中的氨基酸变化。所采用的方法是聚合酶链反应-限制性片段长度多态性（PCR-RFLP）。通过用核酸内切酶III消化β-Lg基因外显子II扩增的PCR产物来检测基因多态性。结果显示扩增产物为247 bp。用III进行限制性消化显示出三种基因型：AA、AB和BB。在谢利菲安牛、舍尔法牛和盖尔莫瓦牛中，AA、AB和BB基因型的基因型频率分别为0.08、0.41、0.50；0.08、0.41、0.50和0.01、0.19、0.56。盖尔莫瓦牛群中不存在AA基因型。在谢利菲安牛和舍尔法牛中，A和B等位基因的频率均为0.29和0.71，在盖尔莫瓦牛群中为0.25和0.75。这些结果进一步证实瘤牛主要为β-乳球蛋白B型。-值<0.05的卡方检验结果显示，谢利菲安牛和舍尔法牛群体处于哈迪-温伯格平衡，而盖尔莫瓦牛群体的结果不显著。这些遗传信息可能有助于评估多态性对阿尔及利亚牛群不同产奶量的影响。
