在噬菌体T5感染大肠杆菌过程中一种膜结合染料的荧光变化。
Fluorescence changes of a membrane-bound dye during bacteriophage T5 infection of Escherichia coli.
作者信息
Glenn J, Duckworth D H
出版信息
J Virol. 1980 Jan;33(1):553-6. doi: 10.1128/JVI.33.1.553-556.1980.
Abstract
The fluorescence intensity of membrane-bound N-phenyl-1-naphthylamine increases dramatically when T5 bacteriophage infect colicin Ib plasmid-containing hosts. This dramatic increase is not seen during normal infections or in infections wherein either the plasmid or the phage contain mutations which allow productive infection to occur. Two smaller increases in fluorescence intensity are seen, however, in all T5 infections in which the characteristic two-step injection of DNA can proceed.
摘要
当T5噬菌体感染含有大肠菌素Ib质粒的宿主时，膜结合的N-苯基-1-萘胺的荧光强度会急剧增加。在正常感染期间或在质粒或噬菌体含有允许产生性感染发生的突变的感染中，不会出现这种急剧增加的情况。然而，在所有能够进行特征性两步DNA注射的T5感染中，会观察到荧光强度有两次较小的增加。
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