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解硫胺素芽孢杆菌温和噬菌体phiBA1的类细菌素杀伤作用

Bacteriocinlike killing action of a temperate bacteriophage phiBA1 of Bacillus aneurinolyticus.

作者信息

Ito S, Nishimune T, Abe M, Kimoto M, Hayashi R

出版信息

J Virol. 1986 Jul;59(1):103-11. doi: 10.1128/JVI.59.1.103-111.1986.

DOI:10.1128/JVI.59.1.103-111.1986
PMID:3086568
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253044/
Abstract

A new temperate phage, phiBA1, was isolated from Bacillus aneurinolyticus, phiBA1 had an icosahedral head with a diameter of about 70 nm and a tail about 20 nm long and contained a circularly permuted, linear duplex DNA of about 38 x 106 daltons. This phage showed two activities: bacteriocin-like killing activity against five strains of B. aneurinolyticus and normal temperate phage activity against three other strains. phiBA1 killed sensitive cells by a single-hit process. After adsorption of phiBA1 to cells sensitive to killing, the content of intracellular ATP increased for the first 5 min and then gradually decreased. Phage DNA injected into the cell immediately after infection was degraded rapidly. Killing was also caused by heavily UV-irradiated phiBA1. Killing-resistant mutants showed normal adsorption of phiBA1 and normal injection of the DNA with its instantaneous restriction. Our results indicate that the killing action of phiBA1 is different from the phenomenon of abortive infection and suggest that the killing might be caused by a proteinaceous component of phiBA1.

摘要

从解硫胺素芽孢杆菌中分离出一种新的温和噬菌体phiBA1，phiBA1具有直径约70nm的二十面体头部和约20nm长的尾部，并且含有一条约38×10⁶道尔顿的环状排列的线性双链DNA。该噬菌体表现出两种活性：对五株解硫胺素芽孢杆菌具有类细菌素杀伤活性，对其他三株菌株具有正常的温和噬菌体活性。phiBA1通过单次打击过程杀死敏感细胞。在phiBA1吸附到对杀伤敏感的细胞后，细胞内ATP含量在最初5分钟内增加，然后逐渐下降。感染后立即注入细胞的噬菌体DNA迅速降解。重度紫外线照射的phiBA1也会导致杀伤。抗杀伤突变体显示phiBA1的正常吸附以及DNA的正常注入及其瞬时限制。我们的结果表明phiBA1的杀伤作用不同于流产感染现象，并表明杀伤可能是由phiBA1的一种蛋白质成分引起的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/3b15eb57520c/jvirol00106-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/a91a5dccc11d/jvirol00106-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/841456b797a7/jvirol00106-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/35dbb114abf5/jvirol00106-0114-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/430c74ed3298/jvirol00106-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/139df62cc8ab/jvirol00106-0115-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/3b15eb57520c/jvirol00106-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/a91a5dccc11d/jvirol00106-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/841456b797a7/jvirol00106-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/35dbb114abf5/jvirol00106-0114-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/430c74ed3298/jvirol00106-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/139df62cc8ab/jvirol00106-0115-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd1/253044/3b15eb57520c/jvirol00106-0117-a.jpg

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本文引用的文献

1
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J Virol. 1969 Sep;4(3):264-70. doi: 10.1128/JVI.4.3.264-270.1969.
2
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.根据脱氧核糖核酸在氯化铯中的浮力密度测定其碱基组成。
J Mol Biol. 1962 Jun;4:430-43. doi: 10.1016/s0022-2836(62)80100-4.
3
CIRCULAR T2 DNA MOLECULES.环状T2 DNA分子。
新型芽孢杆菌噬菌体物种。
Arch Virol. 1994;135(3-4):333-44. doi: 10.1007/BF01310018.
4
Lysis gene t of T-even bacteriophages: evidence that colicins and bacteriophage genes have common ancestors.T-偶数噬菌体的裂解基因t：关于大肠杆菌素和噬菌体基因有共同祖先的证据。
J Bacteriol. 1987 Jul;169(7):2956-61. doi: 10.1128/jb.169.7.2956-2961.1987.
Proc Natl Acad Sci U S A. 1964 Nov;52(5):1297-301. doi: 10.1073/pnas.52.5.1297.
4
The new occurrence of a new pyrimidine base replacing thymine in a bacteriophage DNA:5-hydroxymethyl uracil.在噬菌体DNA中，一种新的嘧啶碱基取代胸腺嘧啶的新情况：5-羟甲基尿嘧啶。
J Mol Biol. 1962 Aug;5:248-50. doi: 10.1016/s0022-2836(62)80087-4.
5
On the purification and some properties of a pyocin, a bacteriocin produced by Pseudomonas aeruginosa.关于绿脓菌素（一种由铜绿假单胞菌产生的细菌素）的纯化及某些性质
Life Sci (1962). 1962 Sep;1:471-6. doi: 10.1016/0024-3205(62)90055-3.
6
Diffusion-in-gel methods for immunological analysis.用于免疫分析的凝胶扩散法。
Prog Allergy. 1958;5:1-78.
7
[Biosynthesis of a colicin and its mode of action].[一种大肠杆菌素的生物合成及其作用方式]
Ann Inst Pasteur (Paris). 1952 Sep;83(3):295-315.
8
Correlation between specific plasmids and delta-endotoxin production in Bacillus thuringiensis.苏云金芽孢杆菌中特定质粒与δ-内毒素产生之间的相关性。
Plasmid. 1981 May;5(3):352-65. doi: 10.1016/0147-619x(81)90010-x.
9
Baseplate protein of bacteriophage T4 with both structural and lytic functions.噬菌体T4的基板蛋白，兼具结构和裂解功能。
J Virol. 1980 Apr;34(1):95-103. doi: 10.1128/JVI.34.1.95-103.1980.
10
Effect of defective phages on the cell membrane of Bacillus subtilis and partial characterization of the phage protein involved in killing.
J Gen Virol. 1981 Oct;56(Pt 2):275-86. doi: 10.1099/0022-1317-56-2-275.