Department of Chemical Engineering and Food Engineering (EQA), Federal University of Santa Catarina (UFSC), Florianópolis, 88040-900, Brazil.
Bioprocess Biosyst Eng. 2017 Sep;40(9):1291-1303. doi: 10.1007/s00449-017-1788-9. Epub 2017 May 24.
The goal of this study was the biosynthesis of silver nanoparticles (SNPs) mediated by the fungus Fusarium oxysporum, as well as the characterization of these nanoparticles including evaluation of the particles size and stability under different processing conditions. The results showed that the biosynthesis produced silver nanoparticles having a mean size of 34 nm and zeta potential values below -30 mV at the conditions used, characterizing the nanoparticles as being stable in suspension. Ultraviolet-visible spectroscopy and flame atomic absorption spectroscopy confirmed the formation of silver nanoparticles and Fourier transform infrared spectroscopy detected the bands corresponding to the binding vibration of amide I and II bands of proteins in addition to the presence of cyclic alkanes, cyclohexane, ethers, and aromatic hydrocarbons. Finally, field emission scanning electron microscopy and transmission electron microscopy revealed the formation of spherical and well-dispersed SNPs.
本研究的目的是通过真菌尖孢镰刀菌介导合成银纳米粒子（SNPs），并对这些纳米粒子进行特性分析，包括评估在不同处理条件下的颗粒尺寸和稳定性。结果表明，在所使用的条件下，生物合成产生了平均粒径为 34nm 的银纳米粒子，且zeta 电位值低于-30mV，表明纳米粒子在悬浮液中稳定。紫外-可见光谱和火焰原子吸收光谱证实了银纳米粒子的形成，傅里叶变换红外光谱检测到了与酰胺 I 和 II 带中蛋白质结合振动相对应的谱带，此外还存在环状烷烃、环己烷、醚和芳烃。最后，场发射扫描电子显微镜和透射电子显微镜揭示了球形和分散良好的 SNPs 的形成。
