Nagashima H, Matsui K, Sawasaki T, Kano Y
Jikken Dobutsu. 1987 Jan;36(1):51-6. doi: 10.1538/expanim1978.36.1_51.
A method of nonsurgical embryo collection in the Shiba goat, a native Japanese miniature goat breeding nonseasonally, was developed. The apparatus used for flushing the uterus was made on the model of the two-way catheter for cows. Embryo collection was performed on days 5 to 7 in 37 females superovulated with PMSG and hCG and resulted in successful recovery of 69 embryos in 19 females (51.4%). The average number of embryos collected from each successful female was 3.6. The recovery rate of embryos calculated on the basis of the number of embryos recovered and corpora lutea observed by culdoscopy in 15 successful females was 89.5%. This nonsurgical method seem to be efficient enough for collecting morulae and blastocysts in Shiba goats.
开发了一种在日本本土非季节性繁殖的小型山羊柴犬山羊中进行非手术胚胎采集的方法。用于冲洗子宫的装置是按照牛用双向导管的模型制作的。对37只经孕马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（hCG）超排的雌性山羊在第5至7天进行胚胎采集，19只雌性山羊成功回收了69枚胚胎（51.4%）。每只成功采集的雌性山羊平均采集到的胚胎数为3.6枚。根据15只成功采集的雌性山羊通过后穹窿镜观察到的回收胚胎数和黄体数计算出的胚胎回收率为89.5%。这种非手术方法似乎足以有效地采集柴犬山羊的桑葚胚和囊胚。
