Pereira R J, Sohnrey B, Holtz W
Institute for Animal Husbandry and Genetics, University of Goettingen, Germany.
J Anim Sci. 1998 Feb;76(2):360-3. doi: 10.2527/1998.762360x.
Embryos were collected transcervically from 38 unanesthetized goats in a standing position. Goats of the Boer breed were superovulated by injecting them with 16 mg of pFSH containing 40% LH. Collection took place 6 d after the last mating. A luteolytic dose of PGF2alpha was injected 16, 8, or 0 h before flushing the uterine lumen. One half of each group received an additional injection of oxytocin just before flushing. The flushing catheter was introduced transcervically, and 24 flushings were performed, with a 2-h pause between the first 12 and the last 12 flushings. Injection of PGF2alpha 16 or 8 h before transcervical embryo collection resulted in a significant increase (P < .001, chi-square test) in embryo recovery (-16 h: 91% with oxytocin, 85% without; -8 h: 91% with oxytocin, 80% without) compared with an injection at the time of collection (52% with oxytocin, 66% without). The recovery rate which was estimated as the percentage of embryos recovered relative to the number of corpora lutea counted endoscopically, was comparable to that achieved by surgical collection. There were no differences among groups with regard to embryo morphology. Embryos were transferred, and healthy kids were born. The nonsurgical collection of caprine embryos may be considered a viable alternative to conventional surgical procedures.
在站立姿势下，经宫颈从38只未麻醉的山羊中采集胚胎。给布尔山羊品种的山羊注射含40%促黄体生成素的16毫克促卵泡素进行超数排卵。在最后一次配种后6天进行采集。在冲洗子宫腔前16、8或0小时注射溶黄体剂量的前列腺素F2α。每组中的一半在冲洗前额外注射催产素。经宫颈插入冲洗导管，进行24次冲洗，前12次冲洗和后12次冲洗之间间隔2小时。与在采集时注射相比（使用催产素时为52%，未使用时为66%），在经宫颈采集胚胎前16或8小时注射前列腺素F2α可显著提高（P <.001，卡方检验）胚胎回收率（-16小时：使用催产素时为91%，未使用时为85%；-8小时：使用催产素时为91%，未使用时为80%）。回收率以回收胚胎数相对于内窥镜检查计数的黄体数的百分比来估计，与手术采集的回收率相当。各组之间胚胎形态无差异。胚胎被移植，健康的羔羊出生。山羊胚胎的非手术采集可被视为传统手术方法的可行替代方案。
