Abid Sobia, Sial Nuzhat, Hanif Muhammad, Usman Abid Hafiz Muhammad, Ismail Amna, Tahir Hanniah
Department of Zoology, The Islamia University of Bahawalpur, Pakistan.
Department of Pharmaceutics, Faculty of Pharmacy Bahauddin Zakariya University, Multan, Pakistan.
Heliyon. 2023 Dec 10;10(1):e23452. doi: 10.1016/j.heliyon.2023.e23452. eCollection 2024 Jan 15.
This study investigates the novel application of Phenyl Boronic Acid Functionalized-Quercetin nanoparticles (PBA-Qt NPs) in the context of antibacterial and diabetic wound healing. The research reveals a multifaceted approach, encompassing physicochemical characterization, antioxidant activity, antibacterial potential, and wound healing efficacy. The purpose of the study was to improve wound healing and antibacterial effects of quercetin and its esterified nanoparticles with phenyl boronic acid (PBA-Qt) compared with phenytoin streptozotocin-induced diabetic rats as a model. PBA-Qt NPs were confirmed using TLC, SEM, and FTIR. They exhibited superior DPPH scavenging (84.2 ± 0.12 %) compared to PBA (59.00 ± 0.18 %) and quercetin (79.02 ± 0.17 %). PBA-Qt showed significant antimicrobial properties with ZOI against Gram-negative (30.34 ± 0.02) and Gram-positive bacteria (25.40 ± 0.03). The MIC for was 1.41 ± 0.03 μg/100 μL, and for , it was 8.25 ± 0.02 μg/100 μL. The MBC against was 4.33 ± 0.02 μg/100 μL, and for , it was 8.25 ± 0.02 μg/100 μL. PBA-Qt NPs reduced MIC for both Gram-positive and Gram-negative bacteria compared to quercetin. They enhanced wound healing by 60-99 % in infected diabetic rats, outperforming phenytoin. PBA-Qt NPs stimulated angiogenesis, tissue repair, and regeneration, improving wound closure. In diabetic and non-diabetic wounds, PBA-Qt NPs demonstrated superior wound contraction and granulation tissue formation. In conclusion, PBA-Qt nanoparticles are promising for treating diabetic chronic wounds due to reduced irritation and enhanced antibacterial and wound-healing properties.
本研究探讨了苯基硼酸功能化槲皮素纳米颗粒（PBA-Qt NPs）在抗菌和糖尿病伤口愈合方面的新应用。该研究揭示了一种多方面的方法，包括物理化学表征、抗氧化活性、抗菌潜力和伤口愈合功效。本研究的目的是与苯妥英链脲佐菌素诱导的糖尿病大鼠模型相比，提高槲皮素及其与苯基硼酸酯化的纳米颗粒（PBA-Qt）的伤口愈合和抗菌效果。使用薄层色谱法（TLC）、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）对PBA-Qt NPs进行了确认。与PBA（59.00±0.18%）和槲皮素（79.02±0.17%）相比，它们表现出更强的二苯基苦味酰基自由基（DPPH）清除能力（84.2±0.12%）。PBA-Qt对革兰氏阴性菌（30.34±0.02）和革兰氏阳性菌（25.40±0.03）表现出显著的抗菌性能，抑菌圈（ZOI）明显。其对[具体细菌1]的最低抑菌浓度（MIC）为1.41±0.03μg/100μL，对[具体细菌2]为8.25±0.02μg/100μL。对[具体细菌1]的最低杀菌浓度（MBC）为4.33±0.02μg/100μL，对[具体细菌2]为8.25±0.02μg/100μL。与槲皮素相比，PBA-Qt NPs降低了革兰氏阳性菌和革兰氏阴性菌的MIC。它们使感染的糖尿病大鼠的伤口愈合提高了60%-99%，优于苯妥英。PBA-Qt NPs刺激血管生成、组织修复和再生，促进伤口闭合。在糖尿病和非糖尿病伤口中，PBA-Qt NPs表现出更好的伤口收缩和肉芽组织形成。总之，由于刺激性降低以及抗菌和伤口愈合性能增强，PBA-Qt纳米颗粒在治疗糖尿病慢性伤口方面具有广阔前景。
