Ekstrand A J, Zech L
Exp Cell Res. 1987 Mar;169(1):262-6. doi: 10.1016/0014-4827(87)90244-8.
The human c-fos proto-oncogene was mapped by in situ hybridization to chromosome 14, band q24.3-q31. As a probe we used 3H-labelled mouse c-fos RNA transcribed in vitro by SP6-RNA-polymerase. Of a total of 40 grains on chromosome 14, 19 (47.5%) were located within the 14q24.3-q31 region (p much less than 0.001). Mechanisms for c-fos to gain transforming ability by a break in a critical part of the gene have recently been described. Thus there are several possibilities for chromosomal aberrations within the 14q24.3-q31 region to be responsible for c-fos deregulation and which may result in neoplastic growth. Such specific aberrations are found in a variety of human neoplasms.
通过原位杂交技术，将人类c-fos原癌基因定位到14号染色体q24.3-q31带。我们使用由SP6-RNA聚合酶体外转录的3H标记的小鼠c-fos RNA作为探针。在14号染色体上总共40个银粒中，有19个（47.5%）位于14q24.3-q31区域内（p远小于0.001）。最近已经描述了c-fos通过基因关键部位的断裂获得转化能力的机制。因此，14q24.3-q31区域内的染色体畸变有几种可能性导致c-fos失调，并可能导致肿瘤生长。在多种人类肿瘤中发现了这种特定的畸变。
