Department of Biology, Indiana University, Bloomington, IN, United States of America.
Department of Life Sciences, School of Natural Sciences, Shiv Nadar Institute of Eminence (SNIoE), Dadri, Uttar Pradesh, India.
PLoS One. 2024 Jan 3;19(1):e0287865. doi: 10.1371/journal.pone.0287865. eCollection 2024.
Drosophila melanogaster larval development relies on a specialized metabolic state that utilizes carbohydrates and other dietary nutrients to promote rapid growth. One unique feature of the larval metabolic program is that Lactate Dehydrogenase (Ldh) activity is highly elevated during this growth phase when compared to other stages of the fly life cycle, indicating that Ldh serves a key role in promoting juvenile development. Previous studies of larval Ldh activity have largely focused on the function of this enzyme at the whole animal level, however, Ldh expression varies significantly among larval tissues, raising the question of how this enzyme promotes tissue-specific growth programs. Here we characterize two transgene reporters and an antibody that can be used to study Ldh expression in vivo. We find that all three tools produce similar Ldh expression patterns. Moreover, these reagents demonstrate that the larval Ldh expression pattern is complex, suggesting the purpose of this enzyme varies across cell types. Overall, our studies validate a series of genetic and molecular reagents that can be used to study glycolytic metabolism in the fly.
黑腹果蝇幼虫的发育依赖于一种特殊的代谢状态，这种代谢状态利用碳水化合物和其他膳食营养物质来促进快速生长。幼虫代谢程序的一个独特特征是，与果蝇生命周期的其他阶段相比，乳酸脱氢酶（Ldh）的活性在这个生长阶段高度升高，这表明 Ldh 在促进幼年发育方面起着关键作用。以前对幼虫 Ldh 活性的研究主要集中在该酶在整体动物水平上的功能，然而，Ldh 在幼虫组织中的表达差异很大，这就提出了一个问题，即这种酶如何促进组织特异性的生长程序。在这里，我们描述了两种转基因报告基因和一种抗体，可用于在体内研究 Ldh 的表达。我们发现这三种工具都产生了相似的 Ldh 表达模式。此外，这些试剂表明，幼虫 Ldh 的表达模式很复杂，这表明该酶在不同的细胞类型中具有不同的功能。总的来说，我们的研究验证了一系列遗传和分子试剂，可用于研究果蝇中的糖酵解代谢。
