Muñoz-Prieto Alberto, Llamas-Amor Eva, Contreras-Aguilar María Dolores, Ayala Ignacio, Martín Cuervo María, Cerón José Joaquín, Hansen Sanni
Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Murcia, Spain.
Department of Animal Medicine, Faculty of Veterinary, University of Extremadura, Avda de la Universidad s/n, 10003 Cáceres, Spain.
Metabolites. 2024 Feb 28;14(3):147. doi: 10.3390/metabo14030147.
Ammonia (NH) and bicarbonate (HCO) have been related to gastric ulcers in humans. Ammonia is considered a possible cause of gastric ulcers, whereas bicarbonate has a protective function. The presence of ulcers in the stomach of horses is defined as Equine Gastric Ulcer Syndrome (EGUS), which is a frequent disease in this species, and it has been associated with changes in saliva composition, such as in analytes related to inflammation, immune system and oxidative stress. The objectives of this study were (1) to perform an analytical validation of two automated spectrophotometric assays, one for ammonia and one for bicarbonate, in the horses' saliva and (2) to evaluate their possible variations with EGUS. Analytical validation of the automated assays for ammonia and bicarbonate in the saliva of horses showed that both assays were precise and accurate. In addition, significantly higher values of ammonia and lower values of bicarbonate were found in the saliva of horses with EGUS compared to healthy horses. It can be concluded that ammonia and bicarbonate can be measured in the saliva of horses and that ammonia increases and bicarbonate decreases in this sample type could be related to the presence of EGUS in this species.
氨（NH）和碳酸氢盐（HCO）与人类胃溃疡有关。氨被认为是胃溃疡的一个可能病因，而碳酸氢盐具有保护作用。马胃中溃疡的存在被定义为马胃溃疡综合征（EGUS），这是该物种的一种常见疾病，并且它与唾液成分的变化有关，例如与炎症、免疫系统和氧化应激相关的分析物。本研究的目的是：（1）对马唾液中氨和碳酸氢盐的两种自动分光光度法进行分析验证，一种用于氨，一种用于碳酸氢盐；（2）评估它们在EGUS情况下可能的变化。对马唾液中氨和碳酸氢盐自动分析方法的验证表明，这两种方法都是精确且准确的。此外，与健康马相比，患有EGUS的马唾液中氨的含量显著更高，而碳酸氢盐的含量更低。可以得出结论，氨和碳酸氢盐可以在马的唾液中进行测量，并且这种样本类型中氨含量增加和碳酸氢盐含量降低可能与该物种中EGUS的存在有关。
