Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
BMC Complement Med Ther. 2024 Jan 4;24(1):20. doi: 10.1186/s12906-023-04329-6.
Zinc selenide nanoparticles (ZnSe) are semiconductor metals of zinc and selenium. ZnSe NPs are advantageous for biomedical and bio-imaging applications due to their low toxicity. ZnSe NPs can be used as a therapeutic agent by synthesizing those using biologically safe methods. As a novel facet of these NPs, plant-based ZnSe NPs were fabricated from an aqueous extract of Rosmarinus officinalis L. (RO extract). Physiochemical analyses such as UV-visible and FTIR spectroscopy, SEM-EDX and TEM Imaging, XRD and DLS-Zeta potential analyses confirmed the biological fabrication of RO-ZnSe NPs. Additionally, Ro-ZnSe NPs were investigated for their bioactivity. There was an apparent peak in the UV-visible spectrum at 398 nm to confirm the presence of ZnSe NPs. FTIR analysis confirmed RO-extract participation in ZnSe NPs synthesis by identifying putative functional groups associated with biomolecules. TEM and SEM analyses revealed that RO-ZnSe NPs have spherical shapes in the range of 90-100 nm. According to XRD and EDX analysis, RO-ZnSe NPs had a crystallite size of 42.13 nm and contain Se and Zn (1:2 ratio). These NPs demonstrated approximately 90.6% antioxidant and antibacterial activity against a range of bacterial strains at 100 µg/ml. Antibiofilm activity was greatest against Candida glabrata and Pseudomonas aeruginosa at 100 g/ml. Accordingly, the IC values for anticancer activity against HTB-9, SW742, and HF cell lines were 14.16, 8.03, and 35.35 g/ml, respectively. In light of the multiple applications for ZnSe NPs, our research indicates they may be an excellent option for biological and therapeutic purposes in treating cancers and infections. Therefore, additional research is required to determine their efficacy.
硒化锌纳米粒子（ZnSe）是锌和硒的半导体金属。由于其低毒性，ZnSe NPs 有利于生物医学和生物成像应用。通过使用生物安全的方法合成这些 NPs，可以将它们用作治疗剂。作为这些 NPs 的一个新方面，已经从迷迭香（RO 提取物）的水提取物中制备了基于植物的 ZnSe NPs。物理化学分析，如紫外可见和傅里叶变换红外光谱、SEM-EDX 和 TEM 成像、XRD 和 DLS-Zeta 电位分析，证实了 RO-ZnSe NPs 的生物合成。此外，还研究了 Ro-ZnSe NPs 的生物活性。在紫外可见光谱中，在 398nm 处有一个明显的峰，以证实 ZnSe NPs 的存在。傅里叶变换红外分析通过鉴定与生物分子相关的假定功能基团，证实了 RO-提取物参与 ZnSe NPs 的合成。TEM 和 SEM 分析表明，RO-ZnSe NPs 的形状为 90-100nm 的球形。根据 XRD 和 EDX 分析，RO-ZnSe NPs 的晶粒度为 42.13nm，并且包含 Se 和 Zn（1:2 比）。这些 NPs 在 100µg/ml 时对一系列细菌菌株表现出约 90.6%的抗氧化和抗菌活性。在 100g/ml 时，对 Candida glabrata 和 Pseudomonas aeruginosa 的抗生物膜活性最大。相应地，HTB-9、SW742 和 HF 细胞系的抗癌活性的 IC 值分别为 14.16、8.03 和 35.35g/ml。鉴于 ZnSe NPs 的多种应用，我们的研究表明它们可能是治疗癌症和感染的生物和治疗目的的绝佳选择。因此，需要进一步研究以确定其疗效。
