Selim Samy, Almuhayawi Mohammed S, Saddiq Amna A, Alruhaili Mohammed H, Saied Ebrahim, Sharaf Mohamed H, Tarabulsi Muyassar K, Al Jaouni Soad K
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia.
Department of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz University, 21589, Jeddah, Saudi Arabia.
Bioresour Bioprocess. 2025 Apr 12;12(1):33. doi: 10.1186/s40643-025-00848-x.
The biosynthesis of bimetallic nanoparticles using plant extracts has garnered significant attention due to their eco-friendly and cost-effective nature. This study aimed to biosynthesize magnesium oxide-zinc oxide nanocomposite (MgO-ZnO nanocomposite) using Pluchea indica leaf extract for the first time, with a focus on characterizing its physicochemical properties and evaluating its biological activities. The biosynthesized MgO-ZnO nanocomposite was fully characterized, revealing an absorbance peak at 300 nm using UV-vis spectroscopy. Transmission electron microscopy (TEM) confirmed particle stability within the size range of 5-35 nm. Cytotoxicity analysis on the Wi 38 normal cell line demonstrated an IC value of 179.13 µg/mL, indicating good biosafety. The nanocomposite exhibited potent anticancer activity, with IC values of 73.61 µg/mL and 31.25 µg/mL against Hep-G2 and MCF-7 cancer cell lines, respectively. Antibacterial assays revealed activity against Klebsiella pneumoniae, Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Candida albicans, with minimum inhibitory concentrations (MICs) ranging from 31.25 to 250 µg/mL. Furthermore, the nanocomposite displayed antioxidant activity with an IC value of 175 µg/mL, as determined by the DPPH assay. In conclusion, the successful synthesis of the MgO-ZnO nanocomposite using P. indica leaf extract demonstrates its potential as a safe and effective agent for concentration-dependent antioxidants, antibacterial, and anticancer applications. This study highlights the versatility of plant-mediated biosynthesis in developing functional nanomaterials for biomedical use.
利用植物提取物生物合成双金属纳米粒子因其环保且经济高效的特性而备受关注。本研究旨在首次使用印度阔苞菊叶提取物生物合成氧化镁-氧化锌纳米复合材料（MgO-ZnO纳米复合材料），重点是表征其物理化学性质并评估其生物活性。对生物合成的MgO-ZnO纳米复合材料进行了全面表征，通过紫外-可见光谱法揭示了在300 nm处的吸收峰。透射电子显微镜（TEM）证实了粒径在5-35 nm范围内的颗粒稳定性。对Wi 38正常细胞系的细胞毒性分析表明IC值为179.13 μg/mL，表明其具有良好的生物安全性。该纳米复合材料表现出强大的抗癌活性，对Hep-G2和MCF-7癌细胞系的IC值分别为73.61 μg/mL和31.25 μg/mL。抗菌试验显示对肺炎克雷伯菌、大肠杆菌、蜡样芽孢杆菌、金黄色葡萄球菌和白色念珠菌有活性，最低抑菌浓度（MIC）范围为31.25至250 μg/mL。此外，通过DPPH测定法确定该纳米复合材料的抗氧化活性IC值为175 μg/mL。总之，使用印度阔苞菊叶提取物成功合成MgO-ZnO纳米复合材料证明了其作为浓度依赖性抗氧化剂、抗菌和抗癌应用的安全有效剂的潜力。本研究突出了植物介导的生物合成在开发用于生物医学的功能性纳米材料方面的多功能性。
