Su Yujie, Qiu Bixia, Chang Cuihua, Li Xin, Zhang Mengqi, Zhou Bei, Yang Yanjun
State Key Laboratory of Food Science and Technology, Jiangnan University Wuxi 214122 PR China.
School of Food Science and Technology, Jiangnan University Lihu Avenue 1800 Wuxi 214122 PR China
RSC Adv. 2018 Feb 7;8(11):6192-6199. doi: 10.1039/c7ra12457k. eCollection 2018 Feb 2.
Magnetic molecular imprinted nanoparticles (MMIPs), combining the progressiveness of magnetic nanoparticles and surface molecular imprinting technology, have attracted increasing attention because of the high efficiency and specificity in isolation and enrichment of the target protein. This study focused on the preparation of bovine hemoglobin MMIPs with bovine hemoglobin (BHb) as the template protein and the molecular imprinted polymer covering the functional magnetic nanoparticles modified with silane and acrylic groups. The physicochemical characteristics as well as the dynamics and isothermal adsorption properties of the generated nanoparticles were investigated to determine their efficiency and specificity in the adsorption of target protein. The maximum adsorption of the target protein was 169.29 mg g at a specific pH, which was much larger than those obtained in some other research reports. MMIPs showed favorable selectivity towards BHb in a mixture of three different proteins. The results indicated the significant effects and broad prospects of MMIPs in the isolation and enrichment of specific proteins in the field of food, medicine and biological research.
磁性分子印迹纳米粒子（MMIPs）结合了磁性纳米粒子和表面分子印迹技术的先进性，因其在目标蛋白分离和富集方面的高效性和特异性而受到越来越多的关注。本研究以牛血红蛋白（BHb）为模板蛋白，制备了覆盖有硅烷和丙烯酸基团修饰的功能磁性纳米粒子的分子印迹聚合物牛血红蛋白MMIPs。研究了所制备纳米粒子的物理化学特性以及动力学和等温吸附性能，以确定它们对目标蛋白吸附的效率和特异性。在特定pH值下，目标蛋白的最大吸附量为169.29 mg/g，远高于其他一些研究报告中的结果。MMIPs在三种不同蛋白质的混合物中对BHb表现出良好的选择性。结果表明MMIPs在食品、医药和生物学研究领域中特定蛋白质的分离和富集中具有显著效果和广阔前景。
