Ahmad Zeeshan, Singh Sukhvinder, Lee Tae Jin, Sharma Ashok, Lydic Todd A, Giri Shailendra, Kumar Ashok
Department of Ophthalmology, Visual and Anatomical Sciences/ Kresge Eye Institute, Wayne State University School of Medicine, Detroit, MI, USA.
Augusta University, Augusta, GA, USA. 4 Department of Ophthalmology, Augusta University, Augusta, GA, USA.
Prostaglandins Other Lipid Mediat. 2024 Apr;171:106806. doi: 10.1016/j.prostaglandins.2023.106806. Epub 2024 Jan 5.
Bacterial endophthalmitis is a blinding infectious disease typically acquired during ocular surgery. We previously reported significant alterations in retinal metabolism during Staphylococcus (S) aureus endophthalmitis. However, the changes in retinal lipid composition during endophthalmitis are unknown. Here, using a mouse model of S. aureus endophthalmitis and an untargeted lipidomic approach, we comprehensively analyzed temporal alterations in total lipids and oxylipin in retina. Our data showed a time-dependent increase in the levels of lipid classes, sphingolipids, glycerolipids, sterols, and non-esterified fatty acids, whereas levels of phospholipids decreased. Among lipid subclasses, phosphatidylcholine decreased over time. The oxylipin analysis revealed increased prostaglandin-E2, hydroxyeicosatetraenoic acids, docosahexaenoic acid, eicosapentaenoic acid, and α-linolenic acid. In-vitro studies using mouse bone marrow-derived macrophages showed increased lipid droplets and lipid-peroxide formation in response to S. aureus infection. Collectively, these findings suggest that S. aureus-infection alters the retinal lipid profile, which may contribute to the pathogenesis of bacterial endophthalmitis.
细菌性眼内炎是一种通常在眼科手术期间获得的致盲性传染病。我们之前报道了金黄色葡萄球菌性眼内炎期间视网膜代谢的显著变化。然而，眼内炎期间视网膜脂质组成的变化尚不清楚。在这里，我们使用金黄色葡萄球菌性眼内炎小鼠模型和非靶向脂质组学方法，全面分析了视网膜中总脂质和氧化脂质的时间变化。我们的数据显示，脂质类别、鞘脂、甘油脂质、固醇和非酯化脂肪酸的水平随时间增加，而磷脂水平下降。在脂质亚类中，磷脂酰胆碱随时间减少。氧化脂质分析显示前列腺素 - E2、羟基二十碳四烯酸、二十二碳六烯酸、二十碳五烯酸和α - 亚麻酸增加。使用小鼠骨髓来源的巨噬细胞进行的体外研究表明，金黄色葡萄球菌感染会导致脂滴和脂质过氧化物形成增加。总体而言，这些发现表明金黄色葡萄球菌感染会改变视网膜脂质谱，这可能有助于细菌性眼内炎的发病机制。
