Ge Lijun, Lu Weibo, Wu Huixiang, Liang Jingjing, Liao Jie, Cheng Keyun, Zeng Xi-Xi, Wang Si-Wei, Wang Zejun, Mao Peiqing, Mao Zhujun, Xue Jing, Tu Yuexing, Shen Qing
Collaborative Innovation Center of Seafood Deep Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China.
Zhejiang Provincial Institute for Food and Drug Control, Hangzhou 310052, China.
Food Chem. 2025 May 1;473:143117. doi: 10.1016/j.foodchem.2025.143117. Epub 2025 Jan 27.
Interspecific lipidological variations in seahorse (Hippocampus), as a functional food resource, profoundly influences its bioefficacy. This study employed untargeted and targeted lipidomics to comprehensively analyse and identify previously uncharacterized lipids in four commercial seahorses. A total of 41 lipid subclasses were discerned, encompassing 1114 and 1219 distinct lipid molecular species in positive and negative ion modes, respectively. Significant interspecific differences were observed in fatty acyls, glycerolipids, phospholipids, saccharolipids, sphingolipids, and sterol lipids across various detection modalities. Triacylglycerols and sphingolipids were dominant lipids in four seahorses. Additionally, 58 oxylipins derived from n-3/n-6 polyunsaturated fatty acids were identified and characterized within seahorses for the first time. Notably, the lined seahorse exhibited a remarkable enrichment in docosahexaenoic acid-derived oxylipins, underscoring its potential as valuable sources of functional lipids. Conclusively, these bioactive lipid profiles were proposed as potential biomarkers for distinguishing different seahorse species and substantiating nutritional values based on multivariate statistical analysis.
作为一种功能性食品资源，海马（Hippocampus）的种间脂质学差异对其生物功效有深远影响。本研究采用非靶向和靶向脂质组学方法，全面分析和鉴定了四种商业海马中以前未表征的脂质。共识别出41个脂质亚类，在正离子模式和负离子模式下分别包含1114种和1219种不同的脂质分子种类。在各种检测方式下，观察到不同种之间在脂肪酰基、甘油脂、磷脂、糖脂、鞘脂和甾醇脂质方面存在显著差异。三酰甘油和鞘脂是四种海马中的主要脂质。此外，首次在海马中鉴定并表征了58种源自n-3/n-6多不饱和脂肪酸的氧化脂质。值得注意的是，斑纹海马在二十二碳六烯酸衍生的氧化脂质中表现出显著富集，凸显了其作为功能性脂质宝贵来源的潜力。最后，基于多变量统计分析，这些生物活性脂质谱被提议作为区分不同海马物种和证实营养价值的潜在生物标志物。
