Dai Jiangpeng, Liu Qiaozhen, Xu Xingyuan, Tan Zhijie, Lin Yuexia, Gao Xiaoxia, Zhu Shuang
School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, China.
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China.
Front Plant Sci. 2023 Dec 22;14:1271689. doi: 10.3389/fpls.2023.1271689. eCollection 2023.
The genus is famous for its high medicinal value. However, the high morphological similarities and unclear interspecific genetic relationships have posed challenges to the classification and identification of species. Here, we newly sequenced six chloroplast genomes of species: , , , , , and . Comparisons among the chloroplast genomes of species showed their conservation in structure, gene content, and order. Ten highly variable loci could be potentially used as specific molecular markers in the identification of species. The third position of codons tended to use A/U base, and natural selection contributed more to the formation of codon usage bias in comparison to mutation pressure. Four genes (L, F, 8, and 2) were detected to be subjected to positive selection. Phylogenetic analysis showed that the genus was a monophyletic group, belonging to the tribe Naucleeae. Moreover, was not a variant of . and were not the same species. The results of the comparative and phylogenetic analysis provide valuable references for further research studies of classification, identification, breeding improvement, and phylogenetic relationships in species.
该属以其高药用价值而闻名。然而，高度的形态相似性和种间遗传关系不明确给该属物种的分类和鉴定带来了挑战。在此，我们新测序了该属6个物种的叶绿体基因组：[物种名称1]、[物种名称2]、[物种名称3]、[物种名称4]、[物种名称5]和[物种名称6]。该属物种叶绿体基因组之间的比较显示出它们在结构、基因含量和顺序上的保守性。10个高变位点有潜力用作该属物种鉴定的特异性分子标记。密码子的第三位倾向于使用A/U碱基，与突变压力相比，自然选择对密码子使用偏好的形成贡献更大。检测到4个基因（L、F、8和2）受到正选择。系统发育分析表明，该属是一个单系类群，属于乌檀族。此外，[物种名称X]不是[物种名称Y]的变种。[物种名称A]和[物种名称B]不是同一物种。比较和系统发育分析结果为该属物种的分类、鉴定、育种改良及系统发育关系的进一步研究提供了有价值的参考。
