Singh Poornima, Pandey Brijesh, Purwar Shalini, Nair Ramakrishnan Madhavan, Pratap Aditya
Department of Biotechnology, School of Life Sciences, Mahatma Gandhi Central University, Motihari, 845 401, India.
Department of Basic and Social Sciences, Banda University of Agriculture and Technology, Banda, 210 001, India.
Sci Rep. 2025 Apr 17;15(1):13284. doi: 10.1038/s41598-025-95331-0.
Subtilisin-like serine proteases (SBTs) are serine proteolytic enzymes that play various roles in plant growth, function and stress responses. Vigna glabrescens, a wild relative of mungbean known to be a potential donor of photo- and thermoperiod insensitivity, was characterized for thermotolerance through reproductive biology and gene expression profiling. Whole-genome sequencing of this species has not yet been performed; hence, genome-wide analysis of this species has not been explored. In the present study, a systematic analysis of SBT-encoding genes in the V. radiata (Vradi_SBT) genome was conducted, with a focus on their response during flower development under different temperature regimes, such as optimum temperature, heat and cold stresses, in Vigna radiata and a wild relative, Vigna glabrescens. Thirty-eight Vradi_SBT genes were identified in the V. radiata genome and were further grouped into five distinct subgroups. The key domain of the SBT peptidase, "peptidase_S8_53," was found in all 38 Vradi_SBT proteins, while 28 of them contained the "peptidase_S8" domain. Additionally, 30 of these proteins contained a maximum of 10 motifs. A total of 22 orthologous genes were identified in Arabidopsis thaliana, whereas paralogous gene pairs were detected as tandemly duplicated genes with V. unguiculata. Cis-acting element analysis revealed that these genes presented more stress-responsive promoter sequences than the other promoters. Furthermore, Vradi_SBT-1.9 was found significantly upregulated under both high- and low-temperature stresses. This study provides insights into SBT-encoding genes and their possible role in flower development and thermotolerance in Vigna species.
枯草杆菌蛋白酶样丝氨酸蛋白酶（SBTs）是丝氨酸蛋白水解酶，在植物生长、功能和应激反应中发挥着多种作用。绿豆的野生近缘种光萼绿豆，已知是光周期和温度不敏感性的潜在供体，通过生殖生物学和基因表达谱分析对其耐热性进行了表征。该物种尚未进行全基因组测序；因此，尚未对该物种进行全基因组分析。在本研究中，对绿豆（Vradi_SBT）基因组中编码SBT的基因进行了系统分析，重点关注它们在不同温度条件下，如最适温度、热胁迫和冷胁迫下，在绿豆及其野生近缘种光萼绿豆花发育过程中的反应。在绿豆基因组中鉴定出38个Vradi_SBT基因，并进一步分为五个不同的亚组。在所有38个Vradi_SBT蛋白中都发现了SBT肽酶的关键结构域“peptidase_S8_53”，其中28个含有“peptidase_S8”结构域。此外，这些蛋白中有30个最多含有10个基序。在拟南芥中总共鉴定出22个直系同源基因，而旁系同源基因对则被检测为与豇豆串联重复的基因。顺式作用元件分析表明，这些基因的启动子序列比其他启动子具有更多的应激反应性。此外，发现Vradi_SBT-1.9在高温和低温胁迫下均显著上调。本研究为编码SBT的基因及其在绿豆属物种花发育和耐热性中的可能作用提供了见解。
