Torrisi M R, Lotti L V, Pavan A, Migliaccio G, Bonatti S
J Cell Biol. 1987 Mar;104(3):733-7. doi: 10.1083/jcb.104.3.733.
Sindbis virus-infected baby hamster kidney (BHK) cells were analyzed by thin section fracture-label. Specific immunolabel with antiviral glycoprotein antibodies was used in conjunction with colloidal gold-conjugated protein A. As we previously reported (Torrisi, M. R., and S. Bonatti, 1985, J. Cell Biol., 101:1300-1306), Sindbis transmembrane glycoproteins are present in the inner nuclear membrane as well as in the outer nuclear membrane, endoplasmic reticulum, Golgi stacks and vesicles, and plasma membranes. Viral glycoproteins located on the inner nuclear membrane resemble those present on the outer membrane in terms of amount, distribution, and preferential partition after fracture. We show in this paper that Sindbis glycoproteins after treatment with cycloheximide are removed from the inner nuclear membrane with the same kinetics as their counterparts present on the outer membrane. This finding strongly suggests that newly synthesized transmembrane glycoproteins may freely diffuse to and from the inner nuclear membrane before entering into the intracellular transport pathway to the plasma membrane.
用超薄切片断裂标记法分析了辛德毕斯病毒感染的幼仓鼠肾（BHK）细胞。抗病毒糖蛋白抗体的特异性免疫标记与胶体金偶联的蛋白A结合使用。正如我们之前所报道的（托里西，M.R.，和S.博纳蒂，1985年，《细胞生物学杂志》，101:1300 - 1306），辛德毕斯跨膜糖蛋白存在于内核膜以及外核膜、内质网、高尔基体堆叠和小泡以及质膜中。位于内核膜上的病毒糖蛋白在数量、分布以及断裂后的优先分配方面与外膜上的糖蛋白相似。我们在本文中表明，用环己酰亚胺处理后的辛德毕斯糖蛋白从内核膜上被去除的动力学与其在外膜上的对应物相同。这一发现强烈表明，新合成的跨膜糖蛋白在进入通向质膜的细胞内运输途径之前可能自由地在内核膜之间扩散。
