冷冻断裂细胞化学：小麦胚凝集素和伴刀豆球蛋白A结合位点在冷冻断裂胰腺细胞上的定位。
Freeze-fracture cytochemistry: localization of wheat-germ agglutinin and concanavalin A binding sites on freeze-fractured pancreatic cells.
作者信息
Pinto da Silva P, Torrisi M R, Kachar B
出版信息
J Cell Biol. 1981 Nov;91(2 Pt 1):361-72. doi: 10.1083/jcb.91.2.361.
Abstract
The combined application of thin-section and critical-point-drying "fracture-label" is used to determine the pattern of distribution and partition of wheat-germ agglutinin and concanavalin A binding sites on the membrane faces of freeze-fractured exocrine and endocrine rat pancreatic cells. Whereas the exoplasmic face of plasma membrane is preferentially labeled by both lectins, the endoplasmic reticulum and nuclear envelope are strongly and uniformly labeled by concanavalin A but not by wheat-germ agglutinin. The results support current views in the glycosylation of membrane proteins and do not support the backflow of sialidated glycoproteins to the endoplasmic reticulum.
摘要
薄切片和临界点干燥“断裂标记”的联合应用被用于确定小麦胚凝集素和伴刀豆球蛋白A结合位点在冷冻断裂的大鼠外分泌和内分泌胰腺细胞膜面上的分布和分配模式。虽然两种凝集素都优先标记质膜的细胞外表面，但内质网和核膜被伴刀豆球蛋白A强烈且均匀地标记，而不被小麦胚凝集素标记。这些结果支持了目前关于膜蛋白糖基化的观点，不支持唾液酸化糖蛋白回流到内质网的观点。
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