细胞内膜糖组分的区室化通过断裂标记得以揭示。
Compartmentalization of intracellular membrane glycocomponents is revealed by fracture-label.
作者信息
Torrisi M R, Pinto da Silva P
出版信息
J Cell Biol. 1984 Jan;98(1):29-34. doi: 10.1083/jcb.98.1.29.
Abstract
We used thin-section fracture-label to determine the distribution of wheat-germ agglutinin binding sites in intracellular membranes of secretory and nonsecretory rat tissues as well as in human leukocytes. In all cases, analysis of the distribution of wheat germ agglutinin led to the definition of two endomembrane compartments: one, characterized by absence of the label, includes the membranes of mitochondria and peroxisomes as well as those of the endoplasmic reticulum and nuclear envelope; the other, strongly labeled, comprises the membrane of lysosomes, phagocytic vacuoles, and secretory granules, as well as the plasma membrane. The Golgi apparatus was weakly labeled in all studied tissues.
摘要
我们使用薄切片断裂标记法来确定小麦胚凝集素结合位点在分泌性和非分泌性大鼠组织以及人类白细胞的细胞内膜中的分布。在所有情况下，对小麦胚凝集素分布的分析都确定了两个内膜区室：一个以无标记为特征，包括线粒体、过氧化物酶体的膜以及内质网和核膜的膜；另一个标记强烈，包括溶酶体、吞噬泡和分泌颗粒的膜以及质膜。在所有研究的组织中，高尔基体标记较弱。
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