Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre s/no., 4169-007 Porto, Portugal.
Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, Portugal.
Int J Syst Evol Microbiol. 2024 Jan;74(1). doi: 10.1099/ijsem.0.006230.
The oceans harbour a myriad of unknown micro-organisms that remain unstudied because of a failure to establish the right growth conditions under laboratory conditions. To overcome this limitation, an isolation effort inspired by the iChip was performed using marine sediments from Memória beach, Portugal. A novel strain, PMIC_1C1B, was obtained and subjected to a polyphasic study. Cells of strain PMIC_1C1B were Gram-positive, rod-shaped, divided by binary fission and formed colonies that were shiny light-yellow. Based on its full 16S rRNA gene sequence, strain PMIC_1C1B was phylogenetically associated to the genus and its closest relatives were KACC 15219 (98.55 %), RZ63 (98.48 %) and JCM 14558 (98.13 %). Strain PMIC_1C1B had a genome size of 2 761 607 bp with 67.71 mol% of G+C content and 2582 coding sequences, which is lower than the genus average. Strain PMIC_1C1B grew from 15 to 30 °C, optimally at 25 °C, at pH 6.0 to 11.0, optimally between pH 6.0 and 8.0, and from 0 to 5 % (w/v) NaCl, optimally between 2.0 and 3.0 %. It grew with casamino acids, glutamine, methionine, -acetylglucosamine, sodium nitrate, tryptophan, urea and valine as sole nitrogen sources, and arabinose and cellobiose as sole carbon sources. The major cellular fatty acids were anteiso-C, iso-C and iso-C. Genome mining revealed the presence of four biosynthetic gene clusters (BGCs) with low similarities to other known BCGs. Based on the polyphasic data, strain PMIC_1C1B is proposed to represent a novel species, for which the name sp. nov. (=CECT 30366=LMG 32350) is proposed.
海洋中蕴藏着无数未知的微生物，由于未能在实验室条件下建立正确的生长条件，这些微生物仍未得到研究。为了克服这一限制，我们受到 iChip 的启发，对来自葡萄牙 Memória 海滩的海洋沉积物进行了分离工作。我们获得了一株新型菌株 PMIC_1C1B，并对其进行了多相研究。菌株 PMIC_1C1B 的细胞呈革兰氏阳性，杆状，通过二分分裂分裂，形成闪亮的浅黄色菌落。根据其全长 16S rRNA 基因序列，菌株 PMIC_1C1B 与属内的其他种聚为一簇，其最近的亲缘种为 KACC 15219（98.55%）、RZ63（98.48%）和 JCM 14558（98.13%）。菌株 PMIC_1C1B 的基因组大小为 2761607bp，G+C 含量为 67.71%mol，有 2582 个编码序列，低于属内的平均水平。菌株 PMIC_1C1B 在 15 至 30°C 之间生长，最佳生长温度为 25°C，在 pH 值 6.0 至 11.0 之间生长，最佳 pH 值在 6.0 与 8.0 之间，在 0 至 5%（w/v）NaCl 之间生长，最佳在 2.0 与 3.0%（w/v）NaCl 之间。它可以利用 casamino acids、glutamine、methionine、-acetylglucosamine、sodium nitrate、tryptophan、urea 和 valine 作为唯一的氮源，以及 arabinose 和 cellobiose 作为唯一的碳源生长。主要的细胞脂肪酸为 anteiso-C、iso-C 和 iso-C。基因组挖掘显示，存在四个生物合成基因簇（BGCs），与其他已知 BGCs 的相似度较低。根据多相数据，菌株 PMIC_1C1B 代表一个新的种，提议将其命名为 sp. nov.（=CECT 30366=LMG 32350）。
