Department of Solar Materials, Helmholtz Centre for Environmental Research GmbH - UFZ, Leipzig, Germany; Department of Microbial Biotechnology, Helmholtz Centre for Environmental Research GmbH - UFZ, Leipzig, Germany.
Department of Solar Materials, Helmholtz Centre for Environmental Research GmbH - UFZ, Leipzig, Germany.
J Biotechnol. 2024 Feb 10;381:76-85. doi: 10.1016/j.jbiotec.2024.01.001. Epub 2024 Jan 6.
The physiology of different Escherichia coli stains was analyzed for growth with glycolate as a potentially promising sustainable sole source of carbon and energy. Different E. coli strains showed large differences regarding lag phases after provision of glycolate. Whereas E. coli W showed fast adaptation, E. coli BW25113, JM101, and BL21 (DE3) needed extensive time for adaption (up to 30 generations) until the attainable µ was reached, which, at 30 °C, amounted to 0.20-0.25 h for all strains. The overexpression of genes encoding glycolate degradation did neither overcome the need for adaptation of E. coli BL21 (DE3) nor improve growth of E. coli W. Rather, high level expression of proteins involved in uptake and initial degradation steps had an adverse effect on growth. Overall, the results show a promising capacity of E. coli strains for growth on glycolate.
分析了不同大肠杆菌菌株利用草酸盐作为有前途的可持续碳和能源唯一来源的生长生理学。不同的大肠杆菌菌株在提供草酸盐后表现出巨大的滞后期差异。虽然大肠杆菌 W 表现出快速的适应能力，但大肠杆菌 BW25113、JM101 和 BL21（DE3）需要大量的时间（最多 30 代）才能适应，直到达到可达到的 µ，在 30°C 下，所有菌株的 µ 值达到 0.20-0.25 h。糖酸降解基因的过表达既不能克服大肠杆菌 BL21（DE3）适应的需要，也不能改善大肠杆菌 W 的生长。相反，参与摄取和初始降解步骤的蛋白质的高水平表达对生长有不利影响。总的来说，这些结果表明大肠杆菌菌株在草酸盐上生长的潜力是有希望的。
