Wiederkehr F, Imfeld H, Vonderschmitt D J
J Clin Chem Clin Biochem. 1986 Dec;24(12):1017-21. doi: 10.1515/cclm.1986.24.12.1017.
Two dimensional gel electrophoresis (2-DE), isoelectric focusing (IEF) and agarose gel electrophoresis (AGE) were used to examine cerebrospinal fluid (CSF) and sera from 22 patients with confirmed multiple sclerosis, 11 patients with probable multiple sclerosis and 20 control patients with non-inflammatory neurological diseases of the central nervous system (CNS). All of the 22 patients with confirmed multiple sclerosis showed abnormal patterns of oligoclonal IgG in all three methods. In the CSF from patients with probable multiple sclerosis, oligoclonal IgG was detectable in 18 percent with AGE, in 72 percent with IEF and 90 percent with 2-DE. No oligoclonal IgG was observed in subjects with non-inflammatory neurological diseases. Many artefacts in IEF, which lead to misinterpretation, are eliminated in the 2-DE system. Based on our observations and this study in particular, it is evident that some patients have IgG changes which can be detected only by 2-DE. The application of research-oriented 2-DE for routine clinical purposes is still limited by its cost and technical complexity.
二维凝胶电泳（2-DE）、等电聚焦（IEF）和琼脂糖凝胶电泳（AGE）被用于检测22例确诊为多发性硬化症的患者、11例可能患有多发性硬化症的患者以及20例患有中枢神经系统（CNS）非炎性神经疾病的对照患者的脑脊液（CSF）和血清。所有22例确诊为多发性硬化症的患者在这三种方法中均显示寡克隆IgG模式异常。在可能患有多发性硬化症的患者的脑脊液中，通过AGE检测到寡克隆IgG的比例为18%，通过IEF为72%，通过2-DE为90%。在患有非炎性神经疾病的受试者中未观察到寡克隆IgG。IEF中许多会导致误判的假象在2-DE系统中被消除。基于我们的观察，尤其是这项研究，很明显有些患者的IgG变化只能通过2-DE检测到。面向研究的2-DE在常规临床应用中仍受其成本和技术复杂性的限制。
