Qingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, Qingdao Agricultural University, Qingdao 266109, P. R. China.
Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, P. R. China.
J Agric Food Chem. 2024 Jan 24;72(3):1592-1606. doi: 10.1021/acs.jafc.3c07416. Epub 2024 Jan 10.
Cuticular wax, a critical defense layer for plants, remains a relatively unexplored factor in rumen fermentation. We investigated the impact of cuticular wax on rumen fermentation using triticale as a model. In total, six wax classes were identified, including fatty acids, aldehydes, alkane, primary alcohol, alkyresorcinol, and β-diketone, with low-bloom lines predominated by 46.05% of primary alcohols and high-bloom lines by 35.64% of β-diketone. Low-wax addition (2.5 g/kg DM) increased the gas production by 19.25% ( < 0.05) and total volatile fatty acids by 6.34% ( > 0.05), and enriched key carbohydrate-fermenting rumen microbes like , , and , when compared to non-wax groups. Metabolites linked to nucleotide metabolism, purine metabolism, and protein/fat digestion in the rumen showed a positive correlation with low-wax, benefiting rumen microbes. This study highlights the intricate interplay among cuticular wax, rumen microbiota, fermentation, and metabolomics in forage digestion, providing insights into livestock nutrition and forage utilization.
角质层蜡质作为植物的重要防御层，在瘤胃发酵中仍然是一个相对未被探索的因素。我们以黑小麦为模型，研究了角质层蜡质对瘤胃发酵的影响。总共鉴定出 6 种蜡质类别，包括脂肪酸、醛、烷烃、伯醇、烷基间苯二酚和β-二酮，低毛穗线主要以 46.05%的伯醇为主，高毛穗线以 35.64%的β-二酮为主。低蜡添加（2.5 g/kg DM）使气体产量增加了 19.25%（<0.05），总挥发性脂肪酸增加了 6.34%（>0.05），与无蜡组相比，富含关键碳水化合物发酵瘤胃微生物，如 、 、 。与核苷酸代谢、嘌呤代谢和蛋白质/脂肪消化相关的代谢物与低蜡呈正相关，有利于瘤胃微生物。本研究强调了角质层蜡质、瘤胃微生物群、发酵和代谢组学在饲料消化中的复杂相互作用，为家畜营养和饲料利用提供了新的见解。
