Marçal Raquel, Sousa Pedro, Marques Ana, Pereira Vitória, Guilherme Sofia, Barreto André, Costas Benjamin, Rocha Rui J M, Pacheco Mário
CESAM-Centre for Environmental and Marine Studies and Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
Riasearch, Lda., 3870-168 Murtosa, Portugal.
Animals (Basel). 2023 Dec 27;14(1):93. doi: 10.3390/ani14010093.
The identification of novel feed materials as a source of functional ingredients is a topical priority in the finfish aquaculture sector. Due to the agrotechnical practices associated and phytochemical profiling, halophytes emerge as a new source of feedstuff for aquafeeds, with the potential to boost productivity and environmental sustainability. Therefore, the present study aimed to assess the potential of incorporation (2.5, 5, and 10%), for 2 months, in the diet of juvenile European seabass, seeking antioxidant (in the liver, gills, and blood) and genoprotective (DNA and chromosomal integrity in blood) benefits. Halophyte inclusion showed no impairments on growth performance. Moreover, a tissue-specific antioxidant improvement was apparent, namely through the GSH-related defense subsystem, but revealing multiple and complex mechanisms. A genotoxic trigger (regarded as a pro-genoprotective mechanism) was identified in the first month of supplementation. A clear protection of DNA integrity was detected in the second month, for all the supplementation levels (and the most prominent melioration at 10%). Overall, these results pointed out a functionality of -supplemented diets and a promising way to improve aquaculture practices, also unraveling a complementary novel, low-value raw material, and a path to its valorization.
在海水鱼类养殖领域，鉴定新型饲料原料作为功能性成分的来源是一个热门的优先事项。由于相关的农业技术实践和植物化学分析，盐生植物成为水产饲料的新饲料来源，具有提高生产力和环境可持续性的潜力。因此，本研究旨在评估在欧洲鲈鱼幼鱼日粮中添加（2.5%、5%和10%）盐生植物2个月的潜力，以寻求抗氧化（在肝脏、鳃和血液中）和基因保护（血液中的DNA和染色体完整性）益处。添加盐生植物对生长性能没有损害。此外，组织特异性抗氧化能力有明显改善，即通过与谷胱甘肽相关的防御子系统，但揭示了多种复杂机制。在补充的第一个月发现了一种基因毒性触发因素（被视为一种促基因保护机制）。在第二个月，对于所有补充水平（10%时改善最为显著）都检测到了对DNA完整性的明显保护。总体而言，这些结果指出了添加盐生植物日粮的功能性以及改善水产养殖实践的一种有前景的方法，同时还揭示了一种互补的新型低价值原料及其增值途径。
