有机和无机硒化合物对饲喂富含肌酸和鱼油日粮的羔羊脾脏脂质组学特征产生影响。

Organic and Inorganic Selenium Compounds Affected Lipidomic Profile of Spleen of Lambs Fed with Diets Enriched in Carnosic Acid and Fish Oil.

作者信息

Białek Małgorzata, Białek Agnieszka, Wojtak Wiktoria, Czauderna Marian

机构信息

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland.

School of Health and Medical Sciences, University of Economics and Human Sciences in Warsaw, Okopowa 59, 01-043 Warsaw, Poland.

出版信息

Animals (Basel). 2023 Dec 29;14(1):133. doi: 10.3390/ani14010133.

DOI:10.3390/ani14010133

PMID:38200864

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10778479/

Abstract

The purpose of our study was to investigate the effect of 0.35 mg Se/kg basal diet (BD) (Se as sodium selenate (Se) and yeast rich in seleno-methionine (Se)) and 0.1% carnosic acid (CA) supplementation to the diet containing 1% fish oil (F-O) and 2% rapeseed oil (R-O) on the contents of fatty acids (FA), malondialdehyde (MDA), tocopherols (Ts), and total cholesterol (TCh) in lambs' spleens. A total of 24 male lambs (4 groups per 6 animals) have been fed: the control diet-the basal diet (BD) enriched in F-O and R-O; the CA diet-BD enriched in F-O, R-O, and CA; the SeCA diet-BD enriched in F-O, R-O, CA, and Se; the SeCA diet-BD enriched in F-O, R-O, CA, and Se. Dietary modifications affected the profiles of saturated (SFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids in spleens. The SeCA and SeCA diets increased the docosapentaenoic acid preference in Δ4-desaturase; hence, a higher content of docosahexaenoic acid was found in the spleens of Se- or Se-treated lambs than in spleens of animals receiving the CA and control diets. The SeCA and SeCA diets increased the concentration ratio of n-3long-chain PUFA (n-3LPUFA) to FA (n-3LPUFA/FA) in spleens compared to the control and CA diets. The content of n-3PUFA was higher in the spleens of Se treated lambs than in spleens of animals receiving the SeCA, CA, and control diets. The SeCA diet increased the content of c9t11CLA in the spleen compared to the control, CA, and SeCA diets. Experimental diets reduced the level of atherogenic FA, the content ratios of n-6PUFA/n-3PUFA and n-6LPUFA/n-3LPUFA, and improved the content ratio of MUFA/FA and the value of the hypocholesterolemic/hypercholesterolemic FA ratio in the spleen in comparison with the control diet. The experimental diets supplemented with Se or Se increased levels of TCh and Ts in spleens in comparison with the CA and control CA diets. The present studies documented that Se, Se, and CA influenced the metabolism of FA, Ts, and cholesterol in spleens.

摘要

我们研究的目的是调查在含1%鱼油（F - O）和2%菜籽油（R - O）的日粮中添加0.35毫克硒/千克基础日粮（BD）（硒以亚硒酸钠（Se）和富含硒代蛋氨酸的酵母（Se）形式存在）以及0.1%肌醇六磷酸（CA）对羔羊脾脏中脂肪酸（FA）、丙二醛（MDA）、生育酚（Ts）和总胆固醇（TCh）含量的影响。总共24只雄性羔羊（每组6只，共4组）被喂食：对照日粮——富含F - O和R - O的基础日粮（BD）；CA日粮——富含F - O、R - O和CA的BD；SeCA日粮——富含F - O、R - O、CA和Se的BD；SeCA日粮——富含F - O、R - O、CA和Se的BD。日粮改良影响了脾脏中饱和脂肪酸（SFA）、单不饱和脂肪酸（MUFA）和多不饱和脂肪酸（PUFA）的分布。SeCA和SeCA日粮增加了Δ4 - 去饱和酶对二十二碳五烯酸的偏好；因此，在接受Se或Se处理的羔羊脾脏中发现的二十二碳六烯酸含量高于接受CA日粮和对照日粮的动物脾脏中的含量。与对照日粮和CA日粮相比，SeCA和SeCA日粮增加了脾脏中n - 3长链多不饱和脂肪酸（n - 3LPUFA）与脂肪酸（n - 3LPUFA/FA）的浓度比。在接受Se处理的羔羊脾脏中，n - 3多不饱和脂肪酸的含量高于接受SeCA、CA和对照日粮的动物脾脏中的含量。与对照日粮、CA日粮和SeCA日粮相比，SeCA日粮增加了脾脏中c9t11CLA的含量。与对照日粮相比，实验日粮降低了致动脉粥样硬化脂肪酸的水平、n - 6多不饱和脂肪酸/n - 3多不饱和脂肪酸和n - 6长链多不饱和脂肪酸/n - 3长链多不饱和脂肪酸的含量比，并改善了脾脏中MUFA/FA的含量比以及降胆固醇/升胆固醇脂肪酸比值。与CA日粮和对照CA日粮相比，添加Se或Se的实验日粮增加了脾脏中TCh和Ts的水平。本研究证明Se、Se和CA影响了脾脏中脂肪酸、Ts和胆固醇的代谢。