Lepionka Tomasz, Białek Małgorzata, Czauderna Marian, Wojtak Wiktoria, Maculewicz Ewelina, Białek Agnieszka
The Biological Threats Identification and Countermeasure Center of the General Karol Kaczkowski Military Institute of Hygiene and Epidemiology, Lubelska 4 St, 24-100 Puławy, Poland.
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland.
Cancers (Basel). 2024 Jan 23;16(3):479. doi: 10.3390/cancers16030479.
The spleen, traditionally associated with blood filtration and immune surveillance, has recently been recognized for its role in systemic lipid metabolism and potential influence on cancer development and progression. This study investigates effects of dietary supplements, specifically conjugated linolenic acids from pomegranate seed oil and bitter melon extract, on the fatty acid (FA) composition of the spleen in the context of cancerous processes. Advanced methods, including gas chromatography-mass spectrometry and silver ion-impregnated high-performance liquid chromatography, were employed to analyze the spleen's FA profile. Our research uncovered that dietary supplementation leads to alterations in the spleen's FA profile, especially under the carcinogenic influence of 7,12-dimethylbenz[a]anthracene. These changes did not align with a simple protective or anti-carcinogenic pattern, as previously suggested in in vitro studies. We observed shifts in conjugated FA isomer concentrations and variations in desaturase activities, suggesting disrupted lipid metabolism in cancerous conditions. The findings underscore the spleen's vital role in lipid metabolism within the body's systemic health framework, highlighting the complexity of dietary supplements' impact on FA profiles in the spleen and their potential implications in cancer progression and treatment. This study adds valuable insight into the complex interplay between diet, disease, and metabolic regulation, particularly in cancerous environments.
传统上，脾脏与血液过滤和免疫监视相关联，最近它在全身脂质代谢中的作用以及对癌症发生和发展的潜在影响也得到了认可。本研究调查了膳食补充剂，特别是石榴籽油中的共轭亚麻酸和苦瓜提取物，在癌症进程背景下对脾脏脂肪酸（FA）组成的影响。采用了先进的方法，包括气相色谱 - 质谱联用和银离子浸渍高效液相色谱法，来分析脾脏的FA谱。我们的研究发现，膳食补充会导致脾脏FA谱发生改变，尤其是在7,12 - 二甲基苯并[a]蒽的致癌影响下。这些变化并不符合先前体外研究中所提出的简单保护或抗癌模式。我们观察到共轭FA异构体浓度的变化以及去饱和酶活性的改变，这表明在癌症状态下脂质代谢受到了干扰。这些发现强调了脾脏在身体整体健康框架内脂质代谢中的重要作用，突出了膳食补充剂对脾脏FA谱影响的复杂性及其在癌症进展和治疗中的潜在意义。这项研究为饮食、疾病和代谢调节之间的复杂相互作用提供了有价值的见解，特别是在癌症环境中。
