Mao Mao, Zhang Zilei, Zhao Xuchong, Geng Haoran, Xue Liang, Liu Danlei
State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Shanghai International Travel Healthcare Center, Shanghai Customs District P. R. China, Shanghai 200335, China.
Foods. 2023 Dec 29;13(1):128. doi: 10.3390/foods13010128.
The prevalence of norovirus in oysters poses a significant threat to food safety, necessitating a comprehensive understanding of contamination patterns. This study explores the temporal dynamics of norovirus distribution in various oyster tissues over a contamination period ranging from 6 to 96 h. Four tissues-the gill, palp, digestive gland, and stomach-were subjected to systematic monitoring using RT-qPCR for absolute quantification. Results revealed rapid norovirus detection in all tissues six hours post-contamination, with subsequent variations in detection rates. Gill and digestive gland tissues exhibited a peak in detection at 12-24 h, aligning with the oyster's gastrointestinal circulatory system. The digestive gland, distinguished by specific enrichment and adsorption capabilities, demonstrated the highest virus concentration at 48 h. In contrast, the stomach displayed a reemergence of norovirus. Beyond 72 h, detection remained exclusive to the digestive gland, with Ct values comparable to earlier time points. At 96 h, a limited amount of norovirus was detected in the digestive gland, emphasizing the importance for timely monitoring. In addition to providing critical insights into optimal detection strategies, these findings highlight the time-related characteristics of norovirus contamination in oysters. The study identifies the digestive gland as a key target for reliable monitoring, providing valuable data to improve protocols for reducing hazards associated with oyster consumption and foodborne norovirus infections. This research contributes to the understanding of norovirus dynamics in oyster tissues and reinforces current efforts aimed at ensuring food safety and public health.
牡蛎中诺如病毒的流行对食品安全构成了重大威胁，因此有必要全面了解污染模式。本研究探讨了在6至96小时的污染期内，诺如病毒在各种牡蛎组织中的时间动态分布。使用RT-qPCR对鳃、触须、消化腺和胃这四种组织进行系统监测以进行绝对定量。结果显示，污染后6小时在所有组织中均快速检测到诺如病毒，随后检测率出现变化。鳃和消化腺组织在12-24小时检测到峰值，这与牡蛎的胃肠循环系统一致。消化腺具有特定的富集和吸附能力，在48小时时病毒浓度最高。相比之下，胃中诺如病毒再次出现。72小时后，仅在消化腺中检测到，其Ct值与早期时间点相当。在96小时时，在消化腺中检测到少量诺如病毒，这强调了及时监测的重要性。这些发现除了为最佳检测策略提供关键见解外，还突出了牡蛎中诺如病毒污染的时间相关特征。该研究确定消化腺是可靠监测的关键目标，提供了有价值的数据以改进减少与食用牡蛎和食源性诺如病毒感染相关危害的方案。这项研究有助于了解诺如病毒在牡蛎组织中的动态，并加强目前旨在确保食品安全和公众健康的努力。
