Valor Diego, García-Casas Ignacio, Montes Antonio, Danese Ella, Pereyra Clara, de la Ossa Enrique Martínez
Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Excellence Agrifood Campus (CeiA3), University of Cádiz, 11510 Puerto Real, Spain.
Faculty of Chemistry and Technology, University of Split, 21000 Split, Croatia.
Polymers (Basel). 2023 Dec 30;16(1):133. doi: 10.3390/polym16010133.
Plant leaves, such as those from , represent a potential utilization of waste due to their richness in bioactive compounds. Supercritical CO allows these compounds to be incorporated into various matrices by impregnation. Combined with its ability to generate polymeric scaffolds, it represents an attractive strategy for the production of biomedical devices. For this purpose, conjugated polymeric scaffolds of biodegradable PLGA (poly(lactic-co-glycolic acid)) and PEDOT:PSS (poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate)), generated in situ by foaming, were employed for the supercritical impregnation of ethanolic mango leaves extract (MLE) in tissue engineering as a potential application. The extraction of MLE was performed by Enhanced Solvent Extraction. The effects of pressure (120-300 bar), temperature (35-55 °C), and depressurization rate (1-50 bar/min) on the physical/conductive properties and the impregnation of MLE were studied. The scaffolds have been characterized by liquid displacement, scanning electron microscope, resistance to conductivity techniques, measurements of impregnated load, antioxidant capacity and antimicrobial activity. Porosity values ranging 9-46% and conductivity values between 10-10 S/cm were obtained. High pressures, low temperatures and rapid depressurization favored the impregnation of bioactive compounds. Scaffolds with remarkable antioxidant activity were obtained (75.2-87.3% oxidation inhibition), demonstrating the ability to inhibit bacterial growth (60.1 to 71.4%).
植物叶子，例如来自[具体植物]的叶子，由于其富含生物活性化合物，代表了一种潜在的废物利用方式。超临界二氧化碳能够通过浸渍将这些化合物融入各种基质中。再结合其生成聚合物支架的能力，这代表了一种用于生产生物医学装置的有吸引力的策略。为此，通过发泡原位生成的可生物降解聚乳酸-乙醇酸共聚物（PLGA）和聚（3,4-亚乙基二氧噻吩）-聚（苯乙烯磺酸盐）（PEDOT:PSS）的共轭聚合物支架，被用于在组织工程中对乙醇芒果叶提取物（MLE）进行超临界浸渍，作为一种潜在应用。MLE的提取通过强化溶剂萃取进行。研究了压力（120 - 300巴）、温度（35 - 55℃）和减压速率（1 - 50巴/分钟）对物理/导电性能以及MLE浸渍的影响。这些支架通过液体置换、扫描电子显微镜、电阻抗技术、浸渍负载测量、抗氧化能力和抗菌活性进行了表征。获得的孔隙率值在9 - 46%之间，电导率值在10 - 10 S/cm之间。高压、低温和快速减压有利于生物活性化合物的浸渍。获得了具有显著抗氧化活性的支架（氧化抑制率为75.2 - 87.3%），证明了其抑制细菌生长的能力（60.1%至71.4%）。
