Grosso Pilar, Cejudo Cristina, Sánchez-Gomar Ismael, Durán-Ruiz Mª Carmen, Moreno-Luna Rafael, Casas Lourdes, Pereyra Clara, Mantell Casimiro
Chemical Engineering and Food Technology Department, Wine and Agrifood Research Institute (IVAGRO), University of Cadiz, Puerto Real, 11519 Cadiz, Spain.
Biomedicine, Biotechnology and Public Health Department, University of Cadiz, 11002 Cadiz, Spain.
Polymers (Basel). 2022 Jul 1;14(13):2706. doi: 10.3390/polym14132706.
The addition of natural substances with pharmacoactive properties to polymeric biomedical devices would provide beneficial regarding the assimilation of these endoprostheses when implanted into a patient's body. The added drug would facilitate endothelization by regulating the inflammatory processes that such interventions entail, preventing contamination hazards and favoring the angiogenesis or formation of blood vessels in the tissue. The present work used mango leaf extract (MLE) obtained through pressurized ethanol for this purpose. Polylactic acid (PLA) in the form of filaments or 3D-printed disks was impregnated by means of supercritical technology with MLE for the culture essays. The release kinetics has been studied and the polymer matrices have been examined by scanning electron microscopy (SEM). The impregnated devices were subjected to in vitro culture of colony-forming endothelial cells. The influence of the different impregnation conditions used for the production of the MLE impregnated polymeric devices on the development of the cell culture was determined by fluorescence microscopy. The best results were obtained from the calcein cultures on 35 °C MLE impregnated into 3D-printed polymer disks.
将具有药理活性的天然物质添加到聚合物生物医学装置中，对于这些植入患者体内的内置假体的同化将是有益的。添加的药物将通过调节此类干预所引发的炎症过程来促进内皮化，防止污染风险，并有利于组织中的血管生成或血管形成。本研究为此使用了通过加压乙醇获得的芒果叶提取物（MLE）。丝状或3D打印圆盘形式的聚乳酸（PLA）通过超临界技术用MLE浸渍以进行培养实验。研究了释放动力学，并通过扫描电子显微镜（SEM）检查了聚合物基质。将浸渍后的装置进行集落形成内皮细胞的体外培养。通过荧光显微镜确定用于生产MLE浸渍聚合物装置的不同浸渍条件对细胞培养发育的影响。在35°C下将MLE浸渍到3D打印聚合物圆盘中进行的钙黄绿素培养获得了最佳结果。
